Work packages
of the GMOchips-project
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Work packagesof the GMOchips-project |
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| Work package name: | Description: |
| WP1: Sequences characterisation |
A. Survey to identify and characterise the plant sequences and the inner sequences of the GMO inserts and their edge fragments. Centralization in a web accessible GMOchips-database. B. Creation of a plasmid bank with cloned components used for the construction of GMOs and plant. C. Definition of the consensus PCR amplification primers. |
| WP2: PCR optimisation |
PCR optimisation on plasmids and DNA extraction. Selection of primers and synthetic internal standards and plant sequences. Detection on biochips. Optimisation on plasmids and genomic DNA. |
| WP3: Low-density biochips |
Design of the low-density biochips: the captures probes and their grafting on the chips, hybridisation optimisation. Design of biochips for expression of plants mRNA specific for the GMOs genes. |
| WP4: Ring-trial |
Ring-trial of the biochips on unknown samples (assesment of the repetability, reproducibility, variability and robustness of the test compared to the owned real-time PCR apparatus of the participating laboratories). |