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Molspec-ID Online Database (MSDB) 1.0

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Database
abrunden

The Project
abrunden

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Taxonomy
Popular Name: Genus: Species: Class: Order: Family:
Animals
Sequence
NCBI-Acc: AB076403 Sequence Description: Bos taurus gene for myostatin, complete cds.
BaseCount: 2196 a 1116 c 1139 g 2209 t
DNA-Sequence: AB076403.txt AminoAcid-Sequence: AB076403.txt
PCR
Name: MY-border Type: Sequencing System: Sequencing Sequence: AB076403
Genetic Element: Myostatin Ampl.Length: 327 GenomSize: AnnTemp: 56
Primers
PPName Type PrimerSequence Length T_ann TmThermo Tm2AT %GC Position
MY-border-f Sense AAACC CAAAT gTTgC TTCTT TAA 23 64.7 56.0 60 30.4 2371-2393
MY-border-r Antisense TgggA AggTT ACAgC AAgAT C 21 63.9 55.1 62 47.6 2697-2677
MY-border-Amplicon Amplicon AAACC CAAAT GTTGC TTCTT TAAat ttagc tctaa gatac aatac aataa actag taaag gccca actgt ggata tatct gaggc ctgtc aagac tcctg cgaca gtgtT TGTGC AAATC CTGAG ACTCA Tcaaa CCCAT GAAAG ACGGT ACAAG GTATA CTGga atccg atctc tgaaa cttga cATGA ACCCA GGCAC TGGTA Tttgg cagag cattg atgtg aagac agtgt tgcag agctg gctca aacaa cctga atcca actta ggcat tgaaa tcaaa gcttt agatg agaat ggcca tGATC TTGCT GTAAC CTTCC CA 327 56 93.2 930 42.2 2371-2697
Organisation Data
Acronym: BfR BfR Phone: +49-30-8412-0
Partner Number: 1 Fax: +41 308412 3685
Type: Authority e-Mail: j.zagon@bfr.bund.de
Name: BfR-Federal Institute for Risk Assessment, Berlin Website: http://www.bfr.bund.de/
Umbrella Organisation: Bund, Germany (Bundesinstitut für Risikobewertung)
Postal Address: Thielallee 88-92, D-14195 Berlin Country: Germany
Remarks: Coordinator MolSpec-ID-project, Task Leader Workpackages 1.4 and 4
Persons
Title / Name e-Mail Phone Remarks
Mr. Hermann Broll h.broll@bfr.bund.de +49-30-8412 3639
Mr. Andreas Butschke a.butschke@bfr.bund.de +49-30-8412 3687
Dr. Jutta Zagon j.zagon@bfr.bund.de +49-30-8412 3876 MolSpec-Coordinator
Samples
FoodName Extraction Process cNucl cDNA aDNA Supplier
Muscle meat from 7 different animal species NucleoSpinTissue NoData 5 - 69 3 BFEL
Muscle meat from 7 different animal species InviSorb NoData 22 - 61 3 BFEL
Muscle meat from 7 different animal species Dneasy NoData 15 - 79 3 BFEL
Muscle meat from 7 different animal species NucleoSpinFood NoData 26 - 132 3 BFEL
Muscle meat from 7 different animal species E.Z.N.A NoData 4 - 26 3 BFEL
Muscle meat from 7 different animal species Lyse NoData 6 - 7 3 BFEL
Muscle meat from 7 different animal species CTAB NoData 83 - 306 3 BFEL
Muscle meat from 7 different animal species Triton-X100 NoData 8 - 23 2 BFEL
Salami (S2 from Partner 2, no heat treatment) CTAB no heat treatment 52±8 80±16 103 VUP
Salami (S2 from Partner 2, no heat treatment) GeneSpin no heat treatment 22±1 42±10 103 VUP
Salami (S2 from Partner 2, no heat treatment) InviSorb no heat treatment 106±17 222±69 104 VUP
Pâté (S3 from Partner 2, heat-treated at 117 °C) CTAB heat-treated at 117 °C 28±7 97±15 102 VUP
Pâté (S3 from Partner 2, heat-treated at 117 °C) GeneSpin heat-treated at 117 °C 6±2 8±7 100 VUP
Pâté (S3 from Partner 2, heat-treated at 117 °C) InviSorb heat-treated at 117 °C 40±13 270±34 102 VUP
Devices
Electrophor. RT-device DNA-Seqencer ThermoCyl. SouthernBlot Other
DNA+protein ABI 7700+LC ABI 310 PE 2400 yes ELISA plate reader
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