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Taxonomy
Popular Name: Genus: Species: Class: Order: Family:
Animals
Sequence
NCBI-Acc: Sequence Description: Sequence-Alignment of 218 Mitochondrial Cytochrome b-Sequences of 218 animal species.
BaseCount:
DNA-Sequence: AminoAcid-Sequence:
PCR
Name: CytB-uni Type: PCR-RFLP System: Sequencing Sequence: Cytochrome b
Genetic Element: Mitochondrial Cytochrome b Ampl.Length: 1143 GenomSize: AnnTemp:
Primers
PPName Type PrimerSequence Length T_ann TmThermo Tm2AT %GC Position
CytB-uni-F Sense CATTT ATTgA CCTCC CAgCC CC 22 70.5 61.7 68 54.5
CytB-uni-R Antisense AgCCT CCTCA gATTC ATTCg AC 22 66.1 57.4 66 50.0
Organisation Data
Acronym: UU-FDG UU-FDG Phone: +31-30-25-0 / +31-30-253 2485
Partner Number: 3 Fax: +31-30-253 6723 /+31 30 2540784
Type: University e-Mail: lenstra@vet.uu.nl
Name: Utrecht University, Institute of Infectious Diseases and Immunology Website: http://www.vet.uu.nl/
Umbrella Organisation: Utrecht University
Postal Address: Institute of Infectious Diseases and Immunology Faculty of Veterinary Medicine UU-Utrecht University Yalelaan 1, PO Box 80165, 3584 CL, Utrecht 3508 TD Utrecht, The Netherlands Country: Netherlands
Remarks: Task Leader Workpackage 1.3
Persons
Title / Name e-Mail Phone Remarks
Dr. J.A. Lenstra lenstra@vet.uu.nl +31-30 2534992
Dr. Ives Nijman i.nijman@vet.uu.nl +31-30-2533943 -
Ms. Lisette van Cann l.vancann@vet.uu.nl +31-30-25-0
Samples
FoodName Extraction Process cNucl cDNA aDNA Supplier
Muscle meat from 7 different animal species NucleoSpinTissue NoData 5 - 69 3 BFEL
Muscle meat from 7 different animal species InviSorb NoData 22 - 61 3 BFEL
Muscle meat from 7 different animal species Dneasy NoData 15 - 79 3 BFEL
Muscle meat from 7 different animal species NucleoSpinFood NoData 26 - 132 3 BFEL
Muscle meat from 7 different animal species E.Z.N.A NoData 4 - 26 3 BFEL
Muscle meat from 7 different animal species Lyse NoData 6 - 7 3 BFEL
Muscle meat from 7 different animal species CTAB NoData 83 - 306 3 BFEL
Muscle meat from 7 different animal species Triton-X100 NoData 8 - 23 2 BFEL
Salami (S2 from Partner 2, no heat treatment) CTAB no heat treatment 52±8 80±16 103 VUP
Salami (S2 from Partner 2, no heat treatment) GeneSpin no heat treatment 22±1 42±10 103 VUP
Salami (S2 from Partner 2, no heat treatment) InviSorb no heat treatment 106±17 222±69 104 VUP
Pâté (S3 from Partner 2, heat-treated at 117 °C) CTAB heat-treated at 117 °C 28±7 97±15 102 VUP
Pâté (S3 from Partner 2, heat-treated at 117 °C) GeneSpin heat-treated at 117 °C 6±2 8±7 100 VUP
Pâté (S3 from Partner 2, heat-treated at 117 °C) InviSorb heat-treated at 117 °C 40±13 270±34 102 VUP
Devices
Electrophor. RT-device DNA-Seqencer ThermoCyl. SouthernBlot Other
yes ABI 7000 ABI 310 ABI 9600 yes
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