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Explanation of the table:  Highly specialised reports on the detection of GMOs in food unavailable in databases

Table 14: Primer sequences and amplicon length in PCR-assays to detect GMOs

Target sequence Primer 1 (5' => 3')
Primer 2 (5' => 3')
Amplicon length (bp) Application (ID) Cycling parameter Ref.
I.) Across interfaces
Structural gene
/ regulatory sequence
antisense PG
P-35S
AGGGGAAAGTGGAAAACCATC
CCACTGACGTAAGGGATGACG
427 Tomato (1) x 12
antisense PG
P-35S
TTTGGAGCTAAGGGTGATGGA
AGTTCATTTCATTTGGAGAGGACA
472 Tomato (12)
 
17
sense PG
P-35S
GAAGATCTGCATGGACCTGAAAA
AGTTCATTTCATTTGGAGAGGACA
478 Tomato (12)
 
17
nptII
P-nos
GAACTCGTCAAGAAGGCGATA
GTTCAAATGCGCCTAAGGTCA
943 Tomato (12) x 17
gene IIIA
nos 3'
CTACTGATTACGGTGCTGCTA
TGAATCCTGTTGCCGGTCTTG
658 Tomato (12) x 17
P-35S
PVX cp
CCACTGACGTAAGGGATGACG
CCAGTTCCATACCACTGGAGC
502 Potato x 5
P-35S
CTP (of CP4 epsps)
TGATGTGATATCTCCACTGACG
TGTATCCCTTGAGCCATGTTGT
172 Soybean (8) x 24
P-35S
dhfr
ATCATTGCGATAAAGGAAAGGC
CTGCCTCCGACTATCCAAACCA
540 Corn x 4
P-35S
dhfr
ATCATTGCGATAAAGGAAAGGC
AAAGCCACAAAAGTCCCAT
840 Corn x 4
CP4 epsps
nos 3'
nda
nda
213/314 Soybean (8) x 13
Regulatory sequence
/ regulatory sequence
P-35S
nos 3'
CAATCCCACTATCCTTCGC
CATCGCAAGACCGGCAACAG
890 Tomato (12) x 17
Structural gene
/ structural gene
cat
lacZ
TTTGTATTCTGAGCATAGTGA
ATAGCGACGAGAGTTAG
623 Microorganism x 10
kat A
cat
CAGCGACTTGAGAAAAACGAGTG
TGTCAGATAGGCCTAATGACTG
1321 Microorganism x 9, 20
nptII
ocd
TATCGCCTTCTTGACGAGTTC
CTGTGGCGGGAACTCCACGA
401 Tomato (12) x 17
ocd
gene IIIA
CGATCCTGAGCGACAATATGA
TAGCAGCACCGTAATCAGTAG
660 Tomato (12) x 17
PVY-cp
nptII
nda
nda
ca. 1800* Potato§ x * 22
II.) Within single
structural genes
als CAGGTCAAGTGGCACGTAGGATG
GGCTGCTTGTTCTTTCCAATCT
642 Cotton 14
aphIV (hygromycin-phosphotransferase) CGCCGATGGTTTCTACAA
GGCGTCGGTTTCCACTAT
839 Potato x 8, 20
barnase CTGGGTGGCATCAAAAGGGAACC
TCCGGTCTGAATTTCTGAAGCCTG
160 Corn (21) x 16
barstar TCAGAAGTATCAGCGACCTCCACC
AAGTATGATGGTGATGTCGCAGCC
235 Corn (21) x 16
gus TCCGTAGAAACCCAACC
GCTAGCCTTGTCCATTG
674 Papaya (26?) x 25
gus ACGTCCTGTAGAAACCCCAA
CCCGCTTCGAAACCAATGCC
1097 Alfalfa x 1
nptII GAACAAGATGGATTGCACGC
GAAGAACTCGTCAAGAAGGC
785 Alfalfa x 1
nptII nda
nda
506 Tom.paste (51, 54) x 11
nptII GGATCTCCTGTCATCT
GATCATCCTGATCGAC
173 Tom (1), Pot, SuB x 12,18
nptII nda
nda
512 Tomato (1) x 19
nptII GGTGCCCTGAATGAACTG
TAGCCAACGCTATGTCCT
ca. 500 Microorganism§ x 21
nptII CTCGACGTTGTCACTGAAGCGGGAAG
AAAGCACGAGGAAGCGGTCAGCCCAT
489b Microorganism§ x 23
nptII CCGACCTGTCCGGTGCCC
CCGCCACACCCAGCCGGCC
475 Soybean (8) x 15
pat (synthetic?) nda
nda
nda Rapeseed§ (66?) 3
pat (synthetic?) nda
nda
552 Corn (15?) 19
pat (synthetic) nda
nda
nda Rapeseed§ (66?) 2
pat (synthetic) nda
nda
532 Corn/Rapeseed§ 6
PG
(polygalacturonase)
CGTTGGTGCATCCCTGCATGG
GGATCCTTAGAAGCATCTAGT
180 (380)a Tomato (12) 17
PG
(polygalacturonase)
nda
nda
427 Tomato (12) 19
II.) Within single
regulatory sequences
P-35S GCTCCTACAAATGCCATCA
195 or 390
195
Tom (1), Soy (8), Corn, SuB, Potato x 18,19
P-35S nda
nda
nda Rapeseed§ (66?) 3
P-TA29 CTTTTTGGTTAGCGAATGC
CTACCATGGTAGCTAATTTC
880 Tobacco x 7
T-nos ( = nos 3') GAATCCTGTTGCCGGTCTTG
TTATCCTAGTTTGCGCGCTA
180 Soybean (8), Pot. x 18

Tom, Tomato; Pot, Potato; SuB, sugarbeet.
* cycling parameters had to be adjusted to the unusual amplicon size.
a) use of these primers resulted in a 380 bp amplicon in control lines, and an additional 180 bp product in transgenic lines (no intron).
b) This amplicon was the result of the second set of primers in a nested PCR; the size of the first product was 753 bp.
§ DNA was extracted from the soil (degraded plant material, or microorganisms).
x Cycling parameter described

References: 1, Blake et al. (1991); 2, Ernst et al. (1996); 3, Feldmann et al. (1996); 4, Golovkin et al. (1993); 5, Jongedijk et al. (1992); 6, Kirchhof et al. (1996); 7, Kriete et al. (1996); 8, LMBG-Methodensammlung (1996); 9, LMBG-Methodensammlung (in press); 10, LMBG-Methodensammlung (in preparation); 11, Meyer, G.; Hanse Analytik, Bremen (personal communication); 12, Meyer (1995a); 13, Padgette et al. (1995); 14, Petition from DuPont for genetically modified cotton (1995); 15, Petition from Monsanto for herbicide-tolerant soybean (1993); 16, Petition from PGS for SeedLink® corn (1995); 17, Petition from Zeneca for genetically modified tomatoes (1995); 18, Pietsch et al. (1997); 19, Pietsch and Waiblinger, (1996); 20, Schulze et al. (1996); 21, Smalla et al. (1993); 22, Stax et al. (1994); 23, Tsushima et al. (1995); 24, Wurz and Willmund, (1997); 25, Yang et al. (1996)


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