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You are here: Core Forms > Search Overview > Overview
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| Taxonomy |
| Popular Name: |
Genus: |
Species: |
Class: |
Order: |
Family: |
|
Wheat |
Triticum |
aestivum L. |
Liliopsida |
Poales |
Poaceae alt. Gramineae |
|
Barley |
Hordeum |
vulgare |
Liliopsida |
Poales |
Pocaceae |
|
Rye |
Secale |
cereale |
Liliopsida |
Poales |
Pocaceae |
| PCR |
| Name: |
WBR |
Type: |
PCR-RFLP |
System: |
QuaLitative (Light Cycler; TaqMan; Matsunaga) |
Sequence: |
|
| Genetic Element: |
Intron of trnL gene |
Ampl.Length: |
|
GenomSize: |
|
AnnTemp: |
|
| Primers |
| PPName |
Type |
PrimerSequence |
Length |
T_ann |
TmThermo |
Tm2AT |
%GC |
Position |
|
WBR11 |
Sense |
ggTAA CTTCC AAATT CAgAg AAAC |
24 |
62.7 |
53.9 |
66 |
37.5 |
|
|
WBR13 |
Antisense |
TCTCT AATTT AgAAT TAgAA ggAA |
24 |
55.7 |
46.9 |
60 |
25.0 |
|
| Organisation Data |
| Acronym: |
VUP
|
|
Phone: |
+421 2 55574622 |
| Partner Number: |
5 |
Fax: |
+421 2 55571417 |
| Type: |
Authority |
e-Mail: |
vup@vup.sk |
| Name: |
VUP-Food Research Institute |
Website: |
http://www.vup.sk/ |
| Umbrella Organisation: |
|
| Postal Address: |
Vyskumny Ustav Potravinarsky
Priemyselna 4
PO Box 25
SK-82475 Bratislava 26, Slovakia |
Country: |
Slovakia |
| Remarks: |
Task Leader Workpackage 1.2 |
| Samples |
| FoodName |
Extraction |
Process |
cNucl |
cDNA |
aDNA |
Supplier |
|
Wheat flour |
CTAB |
flour |
177±36 |
374±20 |
103 |
VUP |
|
Wheat flour |
GeneSpin |
flour |
706±83 |
918±152 |
104 |
VUP |
|
Wheat flour |
InviSorb |
flour |
85±26 |
479±140 |
102 |
VUP |
|
Wheat biscuit |
CTAB |
biscuit |
65±26 |
85±23 |
103 |
VUP |
|
Wheat biscuit |
GeneSpin |
biscuit |
26±3 |
55±2 |
102 |
VUP |
|
Wheat biscuit |
InviSorb |
biscuit |
156±67 |
146±40 |
103 |
VUP |
|
Wheat biscuit with chocolate (Twix) |
CTAB |
biscuit with chocolate (and caramel; Twix) |
34±13 |
31±1 |
102 |
VUP |
|
Wheat biscuit with chocolate (Twix) |
GeneSpin |
biscuit with chocolate (and caramel; Twix) |
26±6 |
23±8 |
102 |
VUP |
|
Wheat biscuit with chocolate (Twix) |
InviSorb |
biscuit with chocolate (and caramel; Twix) |
56±7 |
30±12 |
102 |
VUP |
|
Wheat Brei (Instant) |
CTAB |
Instant Brei |
30±14 |
86±10 |
102 |
VUP |
|
Wheat Brei (Instant) |
GeneSpin |
Instant Brei |
30±3 |
55±1 |
102 |
VUP |
|
Wheat Brei (Instant) |
InviSorb |
Instant Brei |
56±18 |
37±10 |
102 |
VUP |
| Devices |
| Electrophor. |
RT-device |
DNA-Seqencer |
ThermoCyl. |
SouthernBlot |
Other |
|
yes |
ABI 7000 in 2002 |
(only subcontractor) |
ABI 9700 |
(only subcontractor) |
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