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Taxonomy
Popular Name: Genus: Species: Class: Order: Family:
Animals
PCR
Name: DloopSeq Type: Real-Time PCR System: Sequencing Sequence:
Genetic Element: D-Loop Ampl.Length: GenomSize: AnnTemp:
Primers
PPName Type PrimerSequence Length T_ann TmThermo Tm2AT %GC Position
DloopSeq F Sense CCTCT ggTTC CTCgg TCAgg 20 69.5 60.8 66 65.0
DloopSeq R Antisense CCAAA gTgCA TCAgT gTCAA ggTgA T 26 71.9 63.1 76 46.2
Organisation Data
Acronym: TUGraz TUGraz Phone: +43-316 873 6471
Partner Number: 12 Fax: +43-316 8736971
Type: University e-Mail: ursula.muelleder@tugraz.at
Name: Institute of Bio- and Food Chemistry, TUGraz Website: http://www.cis.tugraz.at/ilct/
Umbrella Organisation: University of Technology Graz
Postal Address: TUGraz - Institute of Bio- and Food Chemistry Department of Food Chemistry and Technology Petersgasse 12/II, A-8010, Graz 8010 Graz, Austria Country: Austria
Remarks: Workpackages 1.3, 1.4, 2.1, 3 and 4
Persons
Title / Name e-Mail Phone Remarks
Dr. Ursula Mülleder ursula.muelleder@tugraz.at +43-316 8736499 Replaces Peter Remler in 2002
Dr. Peter Remler peter.remler@tugraz.at +43-316 8736499 Replaced by Ursula Mülleder in 2002
Samples
FoodName Extraction Process cNucl cDNA aDNA Supplier
Muscle meat from 7 different animal species NucleoSpinTissue NoData 5 - 69 3 BFEL
Muscle meat from 7 different animal species InviSorb NoData 22 - 61 3 BFEL
Muscle meat from 7 different animal species Dneasy NoData 15 - 79 3 BFEL
Muscle meat from 7 different animal species NucleoSpinFood NoData 26 - 132 3 BFEL
Muscle meat from 7 different animal species E.Z.N.A NoData 4 - 26 3 BFEL
Muscle meat from 7 different animal species Lyse NoData 6 - 7 3 BFEL
Muscle meat from 7 different animal species CTAB NoData 83 - 306 3 BFEL
Muscle meat from 7 different animal species Triton-X100 NoData 8 - 23 2 BFEL
Salami (S2 from Partner 2, no heat treatment) CTAB no heat treatment 52±8 80±16 103 VUP
Salami (S2 from Partner 2, no heat treatment) GeneSpin no heat treatment 22±1 42±10 103 VUP
Salami (S2 from Partner 2, no heat treatment) InviSorb no heat treatment 106±17 222±69 104 VUP
Pâté (S3 from Partner 2, heat-treated at 117 °C) CTAB heat-treated at 117 °C 28±7 97±15 102 VUP
Pâté (S3 from Partner 2, heat-treated at 117 °C) GeneSpin heat-treated at 117 °C 6±2 8±7 100 VUP
Pâté (S3 from Partner 2, heat-treated at 117 °C) InviSorb heat-treated at 117 °C 40±13 270±34 102 VUP
Devices
Electrophor. RT-device DNA-Seqencer ThermoCyl. SouthernBlot Other
yes ABI 7700 ABI 9600, ABI 9700 yes ELISA plate reader Anthos, UV-VIS Varian
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