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Taxonomy
Popular Name: Genus: Species: Class: Order: Family:
Animals
Sequence
NCBI-Acc: X56295 Sequence Description: S.scrofa mitochondrion cytb gene for cytochrome b.
BaseCount:
DNA-Sequence: X56295.txt AminoAcid-Sequence: X56295.txt
PCR
Name: WP2.2_ELISA-PCR_Myostatin Type: ELISA-PCR System: QuaLitative (Light Cycler; TaqMan; Matsunaga) Sequence: X56295
Genetic Element: Myostatin Ampl.Length: GenomSize: AnnTemp:
Primers
PPName Type PrimerSequence Length T_ann TmThermo Tm2AT %GC Position
MY-r-long Antisense CCAAA TACCA gTgCC TgggT TCA 23 72.1 63.4 70 52.2 2558-2580
BIO_MY-f Sense BIO_T TgTgC AAATC CTgAg ACCAT 21 64.8 55.5 60 42.9 2480-2501
Publications
Short Title Type Date Download
Rep: WP2.2_ELISA_PCR_TibMolbiol Report 2004 Rep_WP2.2_ELISA_PCR_TibMolbiol.pdf
Organisation Data
Acronym: TIB TIB Phone: +39-010-3628388
Partner Number: 11 Fax: +39-010-3621928
Type: Company e-Mail: tib-molbiol@vega.cba.unige.it
Name: Tib Molbiol Website: http://www.tib-molbiol.com/
Umbrella Organisation:
Postal Address: c/o Tib Molbiol S.R.L., Centro Biotecnologie Avanzate, Largo Rosanna Benzi, 10; I-16132, Genova c/o Centro Biotecnologie Avanzate Largo Rosanna Benzi 10 16132 Genova, Italy Largo Rosanna Benzi 10 16132 Genova, Italy Country: Italy
Remarks: Task Leader Workpackage 2.2 and 2.3
Persons
Title / Name e-Mail Phone Remarks
Dr. Davide Bini dbini@tibmolbiol.com; tib-molbiol@vega.cba.unige.it +39-010-3628388
Dr. Elisabetta Carpaneto ecarpaneto@tibmolbiol.com +39-010-3628388
Dr. Olfert Landt OLandt@tib-molbiol.de +49-30-787994-44 Syntheselabor
Dr. Eduardo Thueroff ethueroff@tibmolbiol.com +39-010-3628388
Samples
FoodName Extraction Process cNucl cDNA aDNA Supplier
Muscle meat from 7 different animal species NucleoSpinTissue NoData 5 - 69 3 BFEL
Muscle meat from 7 different animal species InviSorb NoData 22 - 61 3 BFEL
Muscle meat from 7 different animal species Dneasy NoData 15 - 79 3 BFEL
Muscle meat from 7 different animal species NucleoSpinFood NoData 26 - 132 3 BFEL
Muscle meat from 7 different animal species E.Z.N.A NoData 4 - 26 3 BFEL
Muscle meat from 7 different animal species Lyse NoData 6 - 7 3 BFEL
Muscle meat from 7 different animal species CTAB NoData 83 - 306 3 BFEL
Muscle meat from 7 different animal species Triton-X100 NoData 8 - 23 2 BFEL
Salami (S2 from Partner 2, no heat treatment) CTAB no heat treatment 52±8 80±16 103 VUP
Salami (S2 from Partner 2, no heat treatment) GeneSpin no heat treatment 22±1 42±10 103 VUP
Salami (S2 from Partner 2, no heat treatment) InviSorb no heat treatment 106±17 222±69 104 VUP
Pâté (S3 from Partner 2, heat-treated at 117 °C) CTAB heat-treated at 117 °C 28±7 97±15 102 VUP
Pâté (S3 from Partner 2, heat-treated at 117 °C) GeneSpin heat-treated at 117 °C 6±2 8±7 100 VUP
Pâté (S3 from Partner 2, heat-treated at 117 °C) InviSorb heat-treated at 117 °C 40±13 270±34 102 VUP
Devices
Electrophor. RT-device DNA-Seqencer ThermoCyl. SouthernBlot Other
yes ABI 7700, LightCycler yes MS, Biometra, PE, Eppendorf yes ELISA plate reader, MS-spectrometers (Electrospray, Ion Trap, Maldi-TOF)
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