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Primer

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Primer
PPName 12S-border-r Type Antisense PrimerSequence TATgC TTACC TTgTT ACgAC TT
Length 22 T_ann 59.5 TmThermo 50.8 Tm2AT 60 %GC 36.4
Position 1003-982 Sequence Dye ProbeType PCR 12S-border
PCR 12S-border Remarks
PCR
Name: 12S-border Type: Sequencing System: Sequencing Sequence: NC_001941
Genetic Element: 12S rRNA (?) Ampl.Length: 340 GenomSize: AnnTemp: 51
Primers
PPName Type PrimerSequence Length T_ann TmThermo Tm2AT %GC Position
12S-border-f Sense gCTAA TACAg TCTAT ATACC gCC 23 59.5 50.7 66 43.5 664-686
12S-border-Amplicon Amplicon gctaa tacag tctat atacc gccat cttca gcaaa cccta aaaaa gggac aaaag taagc tcaat aataa cacat aaaga cgtta ggtca aggtg taacc tatgg agtgg gaaga aatgg gctac atttt ctacc caaga aaatt taata cgaaa gccat tatga aatta atagc caaag gagga tttag cagta aacta agaat agagt gctta gttga atcag gccat gaagc acgca cacac cgccc gtcac cctcc tcaag taaat atgat atact taaac ctatt tacat atatc aacca cacga gagga gacaa gtcgt aacaa ggtaa gcata 340 51 89.8 942 38.5 664-1003
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