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PPName Ovis-12S-r Type Antisense PrimerSequence CTCTC gTgTg gTTgA TATAT gTAAA
Length 25 T_ann 61.6 TmThermo 52.8 Tm2AT 68 %GC 36.0
Position 976-952 Sequence Dye ProbeType PCR Ovis-12S
PCR Ovis-12S Remarks
Name: Ovis-12S Type: PCR-RFLP System: QuaLitative (Light Cycler; TaqMan; Matsunaga) Sequence: NC_001941
Genetic Element: 12S rRNA (?) Ampl.Length: 193 GenomSize: 3.3 AnnTemp: 64
PPName Type PrimerSequence Length T_ann TmThermo Tm2AT %GC Position
Ovis-12S-f Sense gCTAC ATTTT CTACC CAAgA AAAT 24 62.7 54.0 64 33.3 784-807
Ovis-12S-Amplicon Amplicon gctac atttt ctacc caaga aaatt taata cgaaa gccat tatga aatta atagc caaag gagga tttag cagta aacta agaat agagt gctta gttga atcag gccat gaagc acgca cacac cgccc gtcac cctcc tcaag taaat atgat atact taaac ctatt tacat atatc aacca cacga gag 193 64 87.4 532 37.8 784-976
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