Molspec-ID Online Database (MSDB) 1.0

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PPName Bos-pde-Amplicon Type Amplicon PrimerSequence acaca atgaa ttgAC TCCTA CCCAT CATGC AGATt ctaac atcag gattt ttgct gcatt tgctt catct gtcct tttag agttt tcttT TTTTC TTAGC TGAAA TATTT AAAAA CAaac tccag gcata tcatt GACTC CTCCA TTACG CACC
Length 154 T_ann 60 TmThermo 87.5 Tm2AT 420 %GC 36.4
Position 2994-3147 Sequence Dye ProbeType PCR Bos-pde
PCR Bos-pde Remarks
Name: Bos-pde Type: Real-Time PCR System: QuaNtitative Sequence: M27541
Genetic Element: Phosphodiesterase Ampl.Length: 154 GenomSize: AnnTemp: 60
PPName Type PrimerSequence Length T_ann TmThermo Tm2AT %GC Position
Bos-pde-f Sense ACACA ATgAA TTgAC TCCTA CCC 23 64.1 55.4 66 43.5 2994-3016
Bos-pde-r Antisense ggTgC gTAAT ggAgg AgTC 19 63.1 54.3 60 57.9 3147-3129
Bos-pde-probe Marker/Probe ATATg CCTgg AgTTT gTTTT TAAAT ATTTC AgC 33 71.9 63.1 86 30.3 3124-3092
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