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You are here: Core Froms > Search Primer > Primer
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| Primer |
| PPName |
Gal-Oalb-2r |
Type |
Antisense |
PrimerSequence |
gTCTT gTTgg AAggg ACAgA gTT |
| Length |
23 |
T_ann |
66.5 |
TmThermo |
57.7 |
Tm2AT |
68 |
%GC |
47.8 |
| Position |
8299-8277 |
Sequence |
|
Dye |
|
ProbeType |
|
PCR |
Gal-Oalb-2 |
| PCR |
Gal-Oalb-2 |
Remarks |
|
| PCR |
| Name: |
Gal-Oalb-2 |
Type: |
Real-Time PCR |
System: |
QuaNtitative |
Sequence: |
J00895 |
| Genetic Element: |
Ovalbumin |
Ampl.Length: |
103 |
GenomSize: |
1.25 |
AnnTemp: |
64 |
| Primers |
| PPName |
Type |
PrimerSequence |
Length |
T_ann |
TmThermo |
Tm2AT |
%GC |
Position |
|
Gal-Oalb-2f |
Sense |
TTCTg TATCA AgCAC ATCgC AA |
22 |
65.4 |
56.7 |
62 |
40.9 |
8197-8218 |
|
Gal-Oalb-2probe |
Marker/Probe |
CAACg CCgTT CTCTT CTTTg gCAgA |
25 |
72.1 |
67.4 |
76 |
52.0 |
8220-8244 |
|
Gal-Oalb-2f |
Amplicon |
ttctg tatca agcac ATCGC AACCA ACGCC GTtct cttct ttggc agatg tgttt cccct taaaa agaag aaagc tgaaA AACTC TGTCC CTTCC AACAA GAc |
103 |
64 |
88.8 |
296 |
43.7 |
8197-8299 |
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