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Primer

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Primer
PPName Dloop duck F Type Sense PrimerSequence CAATG CGTIA TCGCG GCATC
Length 20 T_ann 70.6 TmThermo 62.3 Tm2AT 60 %GC 55
Position Sequence Dye ProbeType PCR Dloop duck
PCR Dloop duck Remarks Length: 20; 9th base pair: T or I?
PCR
Name: Dloop duck Type: Real-Time PCR System: QuaNtitative Sequence:
Genetic Element: D-Loop Ampl.Length: GenomSize: 1.36 AnnTemp:
Primers
PPName Type PrimerSequence Length T_ann TmThermo Tm2AT %GC Position
Dloop duck R2 Antisense AAgTC ACTgg gAAgg gCCAg 20 69.6 60.9 64 60.0
Duck-BHQ Marker/Probe TggCg CCTCT ggTTC CTTTT ATTTT TTCC 29 72.1 69.3 84 44.8
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