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abrunden

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abrunden

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Primer

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Primer
PPName Equ_misA_G_A Type Probe_Antisense PrimerSequence FL-gT TAAgA TTTgg AggAT AAggg AgAAT
Length 27 T_ann 66.3 TmThermo 58.5 Tm2AT 74 %GC 37
Position 57-83 Sequence D32190 Dye Fluorescein? ProbeType 25 PCR WP2.2 ELISA-PCR CytochromeB
PCR WP2.2 ELISA-PCR CytochromeB Remarks Cytocrome b. WP 2.2: Development of ELISA-PCR based detection and quantification systems. ELISA-PCR system for cattle, pig sheep and horse. MeltingTempThermo: E. Carpanetto: 58.5 C, TibMolbiol-OligoShop: 57.5 C
PCR
Name: WP2.2 ELISA-PCR CytochromeB Type: ELISA-PCR System: QuaLitative (Light Cycler; TaqMan; Matsunaga) Sequence: X56295
Genetic Element: Mitochondrial Cytochrome b Ampl.Length: GenomSize: AnnTemp:
Primers
PPName Type PrimerSequence Length T_ann TmThermo Tm2AT %GC Position
cons_back_G_ELISA Antisense gTCTg ATgTg TAgTg TATTg CTAgg AATAg g 31 68.1 59.4 88 41.9 144-174
cons_back_M_ELISA Antisense gTCgg ATgTg TAgTg TATTg CTAgg AATAg g 31 71.3 62.5 90 45.2 144-174
BIO_SIM Sense BIO-g ACCTC CCAgC TCCAT CAAAC ATCTC ATCTT gAgAA A 37 72.1 72.4 95 45.9 58-95
Bos_misC Probe_Antisense FL-CC CAggA gggAA CCgCA A 18 71.7 62.9 60 66.7 14609-14626
Sus_misC_C Probe_Antisense FL-TA ggATT TgCAA gATCA ggCCg ATg 25 72.1 64.3 74 48 57-81
Ovi_A_ELISA Probe_Antisense FL-TA gAATC TgTAA AATTA AgCAA ATgCC TAg 30 66.8 58.1 78 30 109-138
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