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PCR

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PCR

Name:

Ros_CSL

Type: Real-Time PCR System: Quantitative
Genetic Element: alcohol dehydrogenase 1 Ampl. Length: 74 Ampl. Position: Sequence Size [bp]:
AnnTemp [°C]: 60 Taxonomy: Rosemary (Rosmarinus officinalis) Temp. Time Program: Process Time [min:s] Temperature [°C]<br>Pre-PCR: decontamination 02:00 50<br>Pre-PCR: activation of DNA polymerase and denaturation of template DNA 10:00 95<br>PCR (45 cycles) <br>Step 1 Denaturation 00:15 95<br>Step 2 Annealing and elongation 01:00 60
Device: Reagents: nPrimers: 3 Organisation: BfR
PCR Description: Sequence:
LOD [copy]: 25 LOQ [copy]: 250 Efficiency [%]: 96
Remarks:

Food/Product

Food Name: Generic Image: Product Type: Food Source: Product Website:
Miel de CorseSUGAR AND HONEY [A0462]INSECT [B1220]http://europa.e... /en/1129_en.htm

Primers

PPName: Type: PrimerSequence: Length [bp]: T_Ann [°C]: TmThermo [°C]: Tm2AT [°C]: GC [%]: Position:
Ros_CSL-f Sense gCg TAT gCg gCC AAC Ag 17 67.7 (PCR: 60) 58.9 56 64.71
Ros_CSL-r Antisense ACT gCC CTT gAA gAA gAA ATg g 22 67.1 (PCR: 60) 58.3 64 45.45
Ros_CSL-probe Marker/Probe 5Ž -FA M)- TG GCA GAT TCA TCA TTC TCC TTC ATT AGG ACC -( TAM RA) -3 32 PCR: 60

Publications (more...)

Short Title Type Date Download
SOP PlantSpecies by PCR SOP September 2007 SOP_BfR_species-detection_071016.pdf
Analytical Methods: Detection of plant species in honey Journal Accepted: 22.09.2008 FoodChemistry_118_979-986_Laube_Honey_2010.pdf

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