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		| PCR | 
	 
	
		| Name: | 
		
			SusT | 
		Type: | 
		
			Real-Time PCR | 
		System: | 
		
			QuaNtitative | 
	 
	
		| Genetic Element: | 
		
			Transferrin | 
		Ampl.Length: | 
		
			78 | 
		GenomSize: | 
		
			3.21 | 
		AnnTemp: | 
		
			58 | 
	 
	
		| Taxonomy: | 
		
			Pig | 
		PCR Description: | 
		
			 | 
		Temp. Time Program: | 
		
			 | 
	 
	
		| Device: | 
		
			 | 
		Master Mix: | 
		
			 | 
		nPrimers: | 
		
			 3 | 
		Organisation: | 
		
			Genescan | 
	 
	
		| Validation: | 
		
			NoValidationAvailable | 
		Sequence: | 
		
			X12386 | 
	 
	
		| Remarks: | 
		
			 | 
	 
 
	
		
			| Primers | 
		 
		
			| PPName | 
			Type | 
			PrimerSequence | 
			Length | 
			T_ann | 
			TmThermo | 
			Tm2AT | 
			%GC | 
			Position | 
		 
				
			| 
				SusTF1 | 
			
				Sense | 
			
				CTggg TCTCA gCgAA ATTCC  | 
			
				 20 | 
			
				 66.7 | 
			
				 57.9 | 
			
				 62 | 
			
				 55.0 | 
			
				  | 
		 
				
			| 
				SusTR1 | 
			
				Antisense | 
			
				TggCT AAgCA TTCCC TTCCA  | 
			
				 20 | 
			
				 67.3 | 
			
				 58.5 | 
			
				 60 | 
			
				 50.0 | 
			
				  | 
		 
				
			| 
				SusTF-MGB | 
			
				Marker/Probe | 
			
				ACgTT CCgAg CCgAT ACA  | 
			
				 18 | 
			
				 65.1 | 
			
				 56.3 | 
			
				 56 | 
			
				 55.6 | 
			
				  | 
		 
			 
	 
	
		| Sequence | 
	 
	
		| NCBI-Acc: | 
		
			X12386 |  
		Sequence Description: | 
		
			Porcine mRNA for transferrin. | 
	 
	
		| BaseCount: | 
		
			621 a 522 c 608 g 504 t | 
	 
	
		| DNA-Sequence: | 
		
			X12386.txt | 
		AminoAcid-Sequence: | 
		
			X12386.txt | 
	 
 
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