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PCR

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PCR
Name: Gal-Oalb Type: Real-Time PCR System: QuaNtitative
Genetic Element: Ovalbumin Ampl.Length: 87 GenomSize: 1.25 AnnTemp: 64
Taxonomy: Chicken PCR Description: Temp. Time Program:
Device: Master Mix: nPrimers: 4 Organisation: BfR
Validation: NoValidationAvailable Sequence: J00895
Remarks: Reference Sequence: J00895 M24999
Primers
PPName Type PrimerSequence Length T_ann TmThermo Tm2AT %GC Position
Gal-Oalb-f Sense ATCgC AACCA ACgCC gT 17 69.4 60.6 54 58.8 8212-8228
Gal-Oalb-r Antisense TCTTg TTggA AgggA CAgAg TTT 23 41.8 57.9 66 43.5 8298-8276
Gal-Oalb-probe Marker/Probe TTTgg CAgAT gTgTT TCCCC TTAAA AAgAA 30 36.9 66.4 82 36.7 8236-8265
Gal-Oalb-f Amplicon ATCGC AACCA ACGCC GTtct cttct ttggc agatg tgttt cccct taaaa agaag aaagc tgaaA AACTC TGTCC CTTCC AACAA GA 87 64 87.8 250 43.7 8212-8298
Sequence
NCBI-Acc: J00895 M24999 Sequence Description: Gallus gallus ovalbumin gene, complete cds.
BaseCount: 2994 a 1749 c 1721 g 2742 t
DNA-Sequence: J00895.txt AminoAcid-Sequence: J00895.txt
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