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		| PCR | 
	 
	
		| Name: | 
		
			BosTF | 
		Type: | 
		
			Real-Time PCR | 
		System: | 
		
			QuaLitative (Light Cycler; TaqMan; Matsunaga) | 
	 
	
		| Genetic Element: | 
		
			Transferrin | 
		Ampl.Length: | 
		
			82 | 
		GenomSize: | 
		
			3.7 | 
		AnnTemp: | 
		
			58 | 
	 
	
		| Taxonomy: | 
		
			Cattle | 
		PCR Description: | 
		
			 | 
		Temp. Time Program: | 
		
			 | 
	 
	
		| Device: | 
		
			 | 
		Master Mix: | 
		
			 | 
		nPrimers: | 
		
			2 | 
		Organisation: | 
		
			Genescan | 
	 
	
		| Validation: | 
		
			NoValidationAvailable | 
		Sequence: | 
		
			U02564 | 
	 
	
		| Remarks: | 
		
			To do: Calculation of Position & Amplicon | 
	 
 
	
		
			| Primers | 
		 
		
			| PPName | 
			Type | 
			PrimerSequence | 
			Length | 
			T_ann | 
			TmThermo | 
			Tm2AT | 
			%GC | 
			Position | 
		 
				
			| 
				BosTF | 
			
				Sense | 
			
				CAAgA ATgCT ACTTg gCAAT gg  | 
			
				 22 | 
			
				 66.7 | 
			
				 57.9 | 
			
				 64 | 
			
				 45.5 | 
			
				  | 
		 
				
			| 
				BosTF | 
			
				Antisense | 
			
				gTTCC CAgAT CACAT CCTCC TT  | 
			
				 22 | 
			
				 65.9 | 
			
				 57.2 | 
			
				 66 | 
			
				 50.0 | 
			
				  | 
		 
			 
	 
	
		| Sequence | 
	 
	
		| NCBI-Acc: | 
		
			U02564 |  
		Sequence Description: | 
		
			Bos taurus transferrin mRNA, complete cds. | 
	 
	
		| BaseCount: | 
		
			667 a    514 c    613 g    544 t | 
	 
	
		| DNA-Sequence: | 
		
			U02564.txt | 
		AminoAcid-Sequence: | 
		
			U02564.txt | 
	 
 
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