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		| PCR | 
	 
	
		| Name: | 
		
			Sltm | 
		Type: | 
		
			Real-Time PCR | 
		System: | 
		
			QuaNtitative | 
	 
	
		| Genetic Element: | 
		
			Lectin | 
		Ampl.Length: | 
		
			 | 
		GenomSize: | 
		
			 | 
		AnnTemp: | 
		
			 | 
	 
	
		| Taxonomy: | 
		
			Soybean | 
		PCR Description: | 
		
			 | 
		Temp. Time Program: | 
		
			 | 
	 
	
		| Device: | 
		
			 | 
		Master Mix: | 
		
			 | 
		nPrimers: | 
		
			3 | 
		Organisation: | 
		
			Nestec | 
	 
	
		| Validation: | 
		
			NoValidationAvailable | 
		Sequence: | 
		
			K00821 | 
	 
	
		| Remarks: | 
		
			Reference Sequence: K00821 M30884.   
Publication: Vaitilingom M, Pijnenburg H, Gendre F and Brignon P (1999): Real-time quantitative PCR detection of genetically modified maximizer maize and RoundupReady soybean in some representative foods.  Journal of Agricultural and Food Chemistry, 47, p. 5261-5266. | 
	 
 
	
		
			| Primers | 
		 
		
			| PPName | 
			Type | 
			PrimerSequence | 
			Length | 
			T_ann | 
			TmThermo | 
			Tm2AT | 
			%GC | 
			Position | 
		 
				
			| 
				Sltm1 | 
			
				Sense | 
			
				AACCg gTAgC gTTgC CAg  | 
			
				 18 | 
			
				 68.5 | 
			
				 59.7 | 
			
				 58 | 
			
				 61.1 | 
			
				 1253-1270 | 
		 
				
			| 
				Sltm2 | 
			
				Antisense | 
			
				AgCCC ATCTg CAAgC CTTT  | 
			
				 19 | 
			
				 68.0 | 
			
				 59.2 | 
			
				 58 | 
			
				 52.6 | 
			
				 1315-1333 | 
		 
				
			| 
				Sltmp | 
			
				Marker/Probe | 
			
				TTCgC CgCTT CCTTC AACTT CACCT  | 
			
				 25 | 
			
				 72.1 | 
			
				 68.1 | 
			
				 76 | 
			
				 52.0 | 
			
				 1272-1296 | 
		 
			 
	 
	
		| Sequence | 
	 
	
		| NCBI-Acc: | 
		
			K00821 |  
		Sequence Description: | 
		
			Soybean lectin (Le1) gene, complete cds. | 
	 
	
		| BaseCount: | 
		
			689 a 417 c 341 g 703 t | 
	 
	
		| DNA-Sequence: | 
		
			K00821.txt | 
		AminoAcid-Sequence: | 
		
			K00821.txt | 
	 
 
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