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		| PCR | 
	 
	
		| Name: | 
		
			PS1 | 
		Type: | 
		
			Real-Time PCR | 
		System: | 
		
			QuaNtitative | 
	 
	
		| Genetic Element: | 
		
			Intergenic spacer region between trnL and trnF exons | 
		Ampl.Length: | 
		
			 | 
		GenomSize: | 
		
			 | 
		AnnTemp: | 
		
			 | 
	 
	
		| Taxonomy: | 
		
			Pea | 
		PCR Description: | 
		
			 | 
		Temp. Time Program: | 
		
			 | 
	 
	
		| Device: | 
		
			 | 
		Master Mix: | 
		
			 | 
		nPrimers: | 
		
			3 | 
		Organisation: | 
		
			Nestec | 
	 
	
		| Validation: | 
		
			NoValidationAvailable | 
		Sequence: | 
		
			 | 
	 
	
		| Remarks: | 
		
			 | 
	 
 
	
		
			| Primers | 
		 
		
			| PPName | 
			Type | 
			PrimerSequence | 
			Length | 
			T_ann | 
			TmThermo | 
			Tm2AT | 
			%GC | 
			Position | 
		 
				
			| 
				PS1for | 
			
				Sense | 
			
				CAAAA gCCAA TTAAA TTCCC TCAT  | 
			
				 24 | 
			
				 66.5 | 
			
				 57.8 | 
			
				 64 | 
			
				 33.3 | 
			
				  | 
		 
				
			| 
				PS1rev | 
			
				Antisense | 
			
				gACgC ACCAT CCTAA TTTTA AgAAA  | 
			
				 25 | 
			
				 66.5 | 
			
				 57.8 | 
			
				 68 | 
			
				 36.0 | 
			
				  | 
		 
				
			| 
				PS1Pb | 
			
				Marker/Probe | 
			
				TTgAT ggCCC TTTTT TgATT TAgTT gACAT AgACT C  | 
			
				 36 | 
			
				 72.1 | 
			
				 67.1 | 
			
				 98 | 
			
				 36.1 | 
			
				  | 
		 
			 
	
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