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| PCR |
| Name: |
BosTF |
Type: |
Real-Time PCR |
System: |
QuaLitative (Light Cycler; TaqMan; Matsunaga) |
| Genetic Element: |
Transferrin |
Ampl.Length: |
82 |
GenomSize: |
3.7 |
AnnTemp: |
58 |
| Taxonomy: |
Cattle |
PCR Description: |
|
Temp. Time Program: |
|
| Device: |
|
Master Mix: |
|
nPrimers: |
2 |
Organisation: |
Genescan |
| Validation: |
NoValidationAvailable |
Sequence: |
U02564 |
| Remarks: |
To do: Calculation of Position & Amplicon |
| Primers |
| PPName |
Type |
PrimerSequence |
Length |
T_ann |
TmThermo |
Tm2AT |
%GC |
Position |
|
BosTF |
Sense |
CAAgA ATgCT ACTTg gCAAT gg |
22 |
66.7 |
57.9 |
64 |
45.5 |
|
|
BosTF |
Antisense |
gTTCC CAgAT CACAT CCTCC TT |
22 |
65.9 |
57.2 |
66 |
50.0 |
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| Sequence |
| NCBI-Acc: |
U02564 |
Sequence Description: |
Bos taurus transferrin mRNA, complete cds. |
| BaseCount: |
667 a 514 c 613 g 544 t |
| DNA-Sequence: |
U02564.txt |
AminoAcid-Sequence: |
U02564.txt |
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