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		| Primer | 
	 
	
		| PPName | 
		
			Gal-Oalb-2probe | 
		Type | 
		
			Marker/Probe | 
		PrimerSequence | 
		
			CAACg CCgTT CTCTT CTTTg gCAgA  | 
	 
	
		| Length | 
		
			 25 | 
		T_ann | 
		
			 72.1 | 
		TmThermo | 
		
			 67.4 | 
		Tm2AT | 
		
			 76 | 
		%GC | 
		
			 52.0 | 
	 
	
		| Position | 
		
			 8220-8244 | 
		Sequence | 
		
			  | 
		Dye | 
		
			  | 
		ProbeType | 
		
			 TaqMan | 
		PCR | 
		
			 Gal-Oalb-2 | 
	 
	
		| PCR | 
		
			 Gal-Oalb-2 | 
		Remarks | 
		
			  | 
	 
 
	
		| PCR | 
	 
	
		| Name: | 
		
			Gal-Oalb-2 | 
		Type: | 
		
			Real-Time PCR | 
		System: | 
		
			QuaNtitative | 
		Sequence: | 
		
			J00895 | 
	 
	
		| Genetic Element: | 
		
			Ovalbumin | 
		Ampl.Length: | 
		
			103 | 
		GenomSize: | 
		
			1.25 | 
		AnnTemp: | 
		
			64 | 
	 
 
	
		
			| Primers | 
		 
		
			| PPName | 
			Type | 
			PrimerSequence | 
			Length | 
			T_ann | 
			TmThermo | 
			Tm2AT | 
			%GC | 
			Position | 
		 
				
			| 
				Gal-Oalb-2f | 
			
				Sense | 
			
				TTCTg TATCA AgCAC ATCgC AA  | 
			
				 22 | 
			
				 65.4 | 
			
				 56.7 | 
			
				 62 | 
			
				 40.9 | 
			
				 8197-8218 | 
		 
				
			| 
				Gal-Oalb-2r | 
			
				Antisense | 
			
				gTCTT gTTgg AAggg ACAgA gTT  | 
			
				 23 | 
			
				 66.5 | 
			
				 57.7 | 
			
				 68 | 
			
				 47.8 | 
			
				 8299-8277 | 
		 
				
			| 
				Gal-Oalb-2f | 
			
				Amplicon | 
			
				ttctg tatca agcac ATCGC AACCA ACGCC GTtct cttct ttggc agatg tgttt cccct taaaa agaag aaagc tgaaA AACTC TGTCC CTTCC AACAA GAc  | 
			
				 103 | 
			
				 64 | 
			
				 88.8 | 
			
				 296 | 
			
				 43.7 | 
			
				 8197-8299 | 
		 
			 
	 
		 | 
	 
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