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PCR

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PCR
Name: Macrop Type: Real-Time PCR System: QuaLitative (Light Cycler; TaqMan; Matsunaga)
Genetic Element: Mitochondrial Cytochrome b Ampl.Length: 164 GenomSize: 4.21/3.13 AnnTemp:
Taxonomy: Kangaroo PCR Description: Temp. Time Program:
Device: Master Mix: nPrimers: 2 Organisation: Eurofins
Validation: NoValidationAvailable Sequence: U87137
Remarks:
Primers
PPName Type PrimerSequence Length T_ann TmThermo Tm2AT %GC Position
Macropf-1 Sense CTTAC CgTTT ATTAT TACAg CCTTA gTAC 29 64.1 55.4 78 34.5
Macropr-1 Antisense ggACA AggAg TATAA gTATA ARgCC A 26 62.4 54.3 70 38.5
Sequence
NCBI-Acc: U87137 Sequence Description: Macropus giganteus cytochrome b (cytb) gene, mitochondrial gene encoding mitochondrial protein, complete cds.
BaseCount: 343 a 363 c 142 g 298 t
DNA-Sequence: U87137.txt AminoAcid-Sequence: U87137.txt
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