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PCR

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PCR
Name: BosTF Type: Real-Time PCR System: QuaLitative (Light Cycler; TaqMan; Matsunaga)
Genetic Element: Transferrin Ampl.Length: 82 GenomSize: 3.7 AnnTemp: 58
Taxonomy: Cattle PCR Description: Temp. Time Program:
Device: Master Mix: nPrimers: 2 Organisation: Genescan
Validation: NoValidationAvailable Sequence: U02564
Remarks: To do: Calculation of Position & Amplicon
Primers
PPName Type PrimerSequence Length T_ann TmThermo Tm2AT %GC Position
BosTF Sense CAAgA ATgCT ACTTg gCAAT gg 22 66.7 57.9 64 45.5
BosTF Antisense gTTCC CAgAT CACAT CCTCC TT 22 65.9 57.2 66 50.0
Sequence
NCBI-Acc: U02564 Sequence Description: Bos taurus transferrin mRNA, complete cds.
BaseCount: 667 a 514 c 613 g 544 t
DNA-Sequence: U02564.txt AminoAcid-Sequence: U02564.txt
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