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PPName Bos-r Type Antisense PrimerSequence TgTTT TTAAA TATTT CAgCT AAgAA AAA
Length 28 T_ann 63.1 TmThermo 54.3 Tm2AT 66 %GC 17.9
Position 3110-3083 Sequence Dye ProbeType PCR Bos-pde0
PCR Bos-pde0 Remarks Primer similar to bosTF; but ca.5bp shift (?)
Name: Bos-pde0 Type: Real-Time PCR System: QuaNtitative Sequence: M27541
Genetic Element: Phosphodiesterase Ampl.Length: 104 GenomSize: AnnTemp: 60
PPName Type PrimerSequence Length T_ann TmThermo Tm2AT %GC Position
Bos-f Sense ACTCC TACCC ATCAT gCAgA T 21 63.1 54.3 62 47.6 3007-3027
Bos-probe Marker/Probe AACAT CAggA TTTTT gCTgC ATTTg C 26 72.1 63.8 72 38.5 3031-3056
Bos-Amplicon Amplicon ACTCC TACCC ATCAT GCAGA Ttcta acatc aggat ttttg ctgca tttgc ttcat ctgtc ctttt agagt tttct tTTTT TCTTA GCTGA AATAT TTAAA AACA 104 60 82.8 276 32.7 3007-3110
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