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PPName Bos-Amplicon Type Amplicon PrimerSequence ACTCC TACCC ATCAT GCAGA Ttcta acatc aggat ttttg ctgca tttgc ttcat ctgtc ctttt agagt tttct tTTTT TCTTA GCTGA AATAT TTAAA AACA
Length 104 T_ann 60 TmThermo 82.8 Tm2AT 276 %GC 32.7
Position 3007-3110 Sequence Dye ProbeType PCR Bos-pde0
PCR Bos-pde0 Remarks
Name: Bos-pde0 Type: Real-Time PCR System: QuaNtitative Sequence: M27541
Genetic Element: Phosphodiesterase Ampl.Length: 104 GenomSize: AnnTemp: 60
PPName Type PrimerSequence Length T_ann TmThermo Tm2AT %GC Position
Bos-f Sense ACTCC TACCC ATCAT gCAgA T 21 63.1 54.3 62 47.6 3007-3027
Bos-r Antisense TgTTT TTAAA TATTT CAgCT AAgAA AAA 28 63.1 54.3 66 17.9 3110-3083
Bos-probe Marker/Probe AACAT CAggA TTTTT gCTgC ATTTg C 26 72.1 63.8 72 38.5 3031-3056
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