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You are here: Core Forms > Search Overview > Overview
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| Taxonomy |
| Popular Name: |
Genus: |
Species: |
Class: |
Order: |
Family: |
|
Soybean |
Glycine |
max |
Rosidae |
Fabales |
Fabaceae (old. Leguminosae. Also placed in: Papilionaceae) |
| Sequence |
| NCBI-Acc: |
K00821 |
Sequence Description: |
Soybean lectin (Le1) gene, complete cds. |
| BaseCount: |
689 a 417 c 341 g 703 t |
| DNA-Sequence: |
K00821.txt |
AminoAcid-Sequence: |
K00821transl.txt |
| PCR |
| Name: |
Sltm |
Type: |
Real-Time PCR |
System: |
QuaNtitative |
Sequence: |
K00821 |
| Genetic Element: |
Lectin |
Ampl.Length: |
|
GenomSize: |
|
AnnTemp: |
|
| Primers |
| PPName |
Type |
PrimerSequence |
Length |
T_ann |
TmThermo |
Tm2AT |
%GC |
Position |
|
Sltm1 |
Sense |
AACCg gTAgC gTTgC CAg |
18 |
68.5 |
59.7 |
58 |
61.1 |
1253-1270 |
|
Sltm2 |
Antisense |
AgCCC ATCTg CAAgC CTTT |
19 |
68.0 |
59.2 |
58 |
52.6 |
1315-1333 |
|
Sltmp |
Marker/Probe |
TTCgC CgCTT CCTTC AACTT CACCT |
25 |
72.1 |
68.1 |
76 |
52.0 |
1272-1296 |
| Organisation Data |
| Acronym: |
Nestec
|
|
Phone: |
+41-21 924 3111/+41 21 7858800
|
| Partner Number: |
14 |
Fax: |
+41-21 7858553 |
| Type: |
Company |
e-Mail: |
etienne.jaccaud@rdls.nestle.com |
| Name: |
Nestec S.A. - Nestlé Research Center Lausanne |
Website: |
http://www.nestle.com/ |
| Umbrella Organisation: |
Néstlé |
| Postal Address: |
Nestlé Research Center Lausanne; Department Quality & Safety Assurance; P.O.Box 44; CH-1000, Lausanne 26
1800 Vevey
Switzerland |
Country: |
Switzerland |
| Remarks: |
Workpackages 1.4, 2.1 and 4 |
| Samples |
| FoodName |
Extraction |
Process |
cNucl |
cDNA |
aDNA |
Supplier |
|
Soya flour (Defatted) |
CTAB |
flour (Defatted) |
224±66 |
141±29 |
102 |
VUP |
|
Soya flour (Defatted) |
GeneSpin |
flour (Defatted) |
2711±309 |
1976±39 |
102 |
VUP |
|
Soya flour (Defatted) |
InviSorb |
flour (Defatted) |
234±35 |
177±19 |
102 |
VUP |
|
Soya biscuit |
CTAB |
biscuit |
49±18 |
61±14 |
101 |
VUP |
|
Soya biscuit |
GeneSpin |
biscuit |
15±8 |
4±0.2 |
100 |
VUP |
|
Soya biscuit |
InviSorb |
biscuit |
78±33 |
50±10 |
101 |
VUP |
|
Soya biscuit with chocolate |
CTAB |
biscuit with chocolate (Margot) |
58±34 |
110±18 |
102 |
VUP |
|
Soya biscuit with chocolate |
GeneSpin |
biscuit with chocolate (Margot) |
81±10 |
94±85 |
102 |
VUP |
|
Soya biscuit with chocolate |
InviSorb |
biscuit with chocolate (Margot) |
67±10 |
88±8 |
102 |
VUP |
| Devices |
| Electrophor. |
RT-device |
DNA-Seqencer |
ThermoCyl. |
SouthernBlot |
Other |
|
yes |
ABI 7700 |
yes |
Biometra Trio-block |
yes |
ELISA plate reader, CE, GeneQuant II Pharmacia, Biochip incubator, PastGel, Western Blot, PAGE |
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