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You are here: Core Forms > Search PCR > PCR PCR  Search all Records  First  Previous Entry No. of 31  Next  Last  PCR Name: WP2.2 ELISA-PCR CytochromeB Type: ELISA-PCR System: QuaLitative (Light Cycler; TaqMan; Matsunaga) Genetic Element: Mitochondrial Cytochrome b Ampl.Length: GenomSize: AnnTemp: Taxonomy: Animals PCR Description: WP 2.2: Development of ELISA-PCR based detection and quantification systems. ELISA-PCR system for cattle, pig sheep and horse Target Element: Cytochrome b Temp. Time Program: PCR profile Taq Activation 95 °C 120 sec Cycles 25 Denat. Amplif. Extension ------------------------------------------------- Target Temp. (°C) 95 62 72 Incubation time (sec) 30 20 30 Final extension 72°C 120 sec Device: Master Mix: nPrimers: Organisation: TIB Validation: Sequence: X56295 Remarks: Detection of Cattle (66), Pig (79), Sheep and Horse. Target Element: Cytochrome b Primers PPName Type PrimerSequence Length T_ann TmThermo Tm2AT %GC Position BIO_SIM Sense BIO-g ACCTC CCAgC TCCAT CAAAC ATCTC ATCTT gAgAA A 37 72.1 72.4 95 45.9 58-95 cons_back_M_ELISA Antisense gTCgg ATgTg TAgTg TATTg CTAgg AATAg g 31 71.3 62.5 90 45.2 144-174 cons_back_G_ELISA Antisense gTCTg ATgTg TAgTg TATTg CTAgg AATAg g 31 68.1 59.4 88 41.9 144-174 Bos_misC Probe_Antisense FL-CC CAggA gggAA CCgCA A 18 71.7 62.9 60 66.7 14609-14626 Sus_misC_C Probe_Antisense FL-TA ggATT TgCAA gATCA ggCCg ATg 25 72.1 64.3 74 48 57-81 Ovi_A_ELISA Probe_Antisense FL-TA gAATC TgTAA AATTA AgCAA ATgCC TAg 30 66.8 58.1 78 30 109-138 Equ_misA_G_A Probe_Antisense FL-gT TAAgA TTTgg AggAT AAggg AgAAT 27 66.3 58.5 74 37 57-83 Sequence NCBI-Acc: X56295 Sequence Description: S.scrofa mitochondrion cytb gene for cytochrome b. BaseCount: DNA-Sequence: X56295.txt AminoAcid-Sequence: X56295transl.txt Publications Short Title Type Date Download Rep: WP2.2_ELISA_PCR_TibMolbiol Report 2004 Rep_WP2.2_ELISA_PCR_TibMolbiol.pdf You are here: Core Forms > Search PCR > PCR © Copyright MolSpec Disclaimer: MolSpec is not responsible for linked contents.