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PCR

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PCR
Name: CytB-uni Type: PCR-RFLP System: Sequencing
Genetic Element: Mitochondrial Cytochrome b Ampl.Length: 1143 GenomSize: AnnTemp:
Taxonomy: Animals PCR Description: Temp. Time Program:
Device: Master Mix: nPrimers: 2 Organisation: UU-FDG
Validation: NoValidationAvailable Sequence: Cytochrome b
Remarks:
Primers
PPName Type PrimerSequence Length T_ann TmThermo Tm2AT %GC Position
CytB-uni-F Sense CATTT ATTgA CCTCC CAgCC CC 22 70.5 61.7 68 54.5
CytB-uni-R Antisense AgCCT CCTCA gATTC ATTCg AC 22 66.1 57.4 66 50.0
Sequence
NCBI-Acc: Sequence Description: Sequence-Alignment of 218 Mitochondrial Cytochrome b-Sequences of 218 animal species.
BaseCount:
DNA-Sequence: AminoAcid-Sequence:
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