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Name: Bos-pde0 Type: Real-Time PCR System: QuaNtitative
Genetic Element: Phosphodiesterase Ampl.Length: 104 GenomSize: AnnTemp: 60
Taxonomy: Cattle PCR Description: Temp. Time Program:
Device: Master Mix: nPrimers: 4 Organisation: BfR
Validation: NoValidationAvailable Sequence: M27541
Remarks: Corr.Primers: bos-f, bos-r and bos-probe. Reference Sequence: M27541
PPName Type PrimerSequence Length T_ann TmThermo Tm2AT %GC Position
Bos-f Sense ACTCC TACCC ATCAT gCAgA T 21 63.1 54.3 62 47.6 3007-3027
Bos-r Antisense TgTTT TTAAA TATTT CAgCT AAgAA AAA 28 63.1 54.3 66 17.9 3110-3083
Bos-probe Marker/Probe AACAT CAggA TTTTT gCTgC ATTTg C 26 72.1 63.8 72 38.5 3031-3056
Bos-Amplicon Amplicon ACTCC TACCC ATCAT GCAGA Ttcta acatc aggat ttttg ctgca tttgc ttcat ctgtc ctttt agagt tttct tTTTT TCTTA GCTGA AATAT TTAAA AACA 104 60 82.8 276 32.7 3007-3110
NCBI-Acc: M27541 Sequence Description: Bovine cyclic GMP phosphodiesterase alpha subunit mRNA, complete cds.
BaseCount: 887 a 783 c 798 g 733 t
DNA-Sequence: M27541.txt AminoAcid-Sequence: M27541.txt
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