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		| Primer | 
	 
	
		| PPName | 
		
			cons_back_M | 
		Type | 
		
			Antisense | 
		PrimerSequence | 
		
			gTCgg ATgTg TAgTg TATTg CTAgg AATAg g  | 
	 
	
		| Length | 
		
			 31 | 
		T_ann | 
		
			 71.3 | 
		TmThermo | 
		
			 62.5 | 
		Tm2AT | 
		
			 90 | 
		%GC | 
		
			 45.2 | 
	 
	
		| Position | 
		
			 144-174 | 
		Sequence | 
		
			 X56295 | 
		Dye | 
		
			  | 
		ProbeType | 
		
			  | 
		PCR | 
		
			 WP2.1QuaL-Multiplex RealTime-PCRCytochromeB | 
	 
	
		| PCR | 
		
			 WP2.1QuaL-Multiplex RealTime-PCRCytochromeB | 
		Remarks | 
		
			 Cytochrome b (Amplicon length: 117bp).
WP 2.1: Development of multiplex-PCR for species in food.
Qualitative multiplex real time PCR for pig, cattle and myostatin on the Light Cycler, Roche. | 
	 
 
	
		
			| Primers | 
		 
		
			| PPName | 
			Type | 
			PrimerSequence | 
			Length | 
			T_ann | 
			TmThermo | 
			Tm2AT | 
			%GC | 
			Position | 
		 
				
			| 
				cons_back_G | 
			
				Antisense | 
			
				gTCTg ATgTg TAgTg TATTg CTAgg AATAg g  | 
			
				 31 | 
			
				 68.1 | 
			
				 59.4 | 
			
				 88 | 
			
				 41.9 | 
			
				 144-174 | 
		 
				
			| 
				SIM_F_iFL | 
			
				Sense | 
			
				gACCT CCCAg CTCCA TCAAA CATCT CATCT TgA X T gAA A  | 
			
				 38 | 
			
				 72.1 | 
			
				 72.4 | 
			
				 95 | 
			
				 44.7 | 
			
				 58-95 | 
		 
				
			| 
				bos_probe_new | 
			
				Probe_Antisense | 
			
				CCCAg gAggg AACCg AAATT  X  | 
			
				 20 | 
			
				 69.6 | 
			
				 60.8 | 
			
				 62 | 
			
				 55 | 
			
				 14607-14627 | 
		 
				
			| 
				sus_probe | 
			
				Probe_Antisense | 
			
				CAgAT gCCTA AgAgg gAACC gA X  | 
			
				 22 | 
			
				 69.3 | 
			
				 60.6 | 
			
				 68 | 
			
				 54.5 | 
			
				 98-119 | 
		 
			 
	 
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