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You are here: Core Froms > Search Primer > Primer Primer  Search all Records  First  Previous Entry No. of 99  Next  Last  Primer PPName SIM_F_iFL Type Sense PrimerSequence gACCT CCCAg CTCCA TCAAA CATCT CATCT TgA X T gAA A Length 38 T_ann 72.1 TmThermo 72.4 Tm2AT 95 %GC 44.7 Position 58-95 Sequence X56295 Dye Fluorescein ProbeType 25 PCR WP2.1QuaL-Multiplex RealTime-PCRCytochromeB PCR WP2.1QuaL-Multiplex RealTime-PCRCytochromeB Remarks XT = “T” internally labelled with Fluorescein (*) modified from Matsunaga et al., 1991. Cytochrome b (Amplicon length: 117bp). WP 2.1: Development of multiplex-PCR for species in food. Qualitative multiplex real time PCR for pig, cattle and myostatin on the Light Cycler, Roche. PCR Name: WP2.1QuaL-Multiplex RealTime-PCRCytochromeB Type: Real-Time PCR + Multiplex System: QuaLitative (Light Cycler; TaqMan; Matsunaga) Sequence: X56295 Genetic Element: Mitochondrial Cytochrome b Ampl.Length: 117 GenomSize: AnnTemp: Primers PPName Type PrimerSequence Length T_ann TmThermo Tm2AT %GC Position cons_back_G Antisense gTCTg ATgTg TAgTg TATTg CTAgg AATAg g 31 68.1 59.4 88 41.9 144-174 cons_back_M Antisense gTCgg ATgTg TAgTg TATTg CTAgg AATAg g 31 71.3 62.5 90 45.2 144-174 bos_probe_new Probe_Antisense CCCAg gAggg AACCg AAATT X 20 69.6 60.8 62 55 14607-14627 sus_probe Probe_Antisense CAgAT gCCTA AgAgg gAACC gA X 22 69.3 60.6 68 54.5 98-119 You are here: Core Froms > Search Primer > Primer © Copyright MolSpec Disclaimer: MolSpec is not responsible for linked contents.