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PPName sus_probe Type Probe_Antisense PrimerSequence CAgAT gCCTA AgAgg gAACC gA X
Length 22 T_ann 69.3 TmThermo 60.6 Tm2AT 68 %GC 54.5
Position 98-119 Sequence X56295 Dye LightCycler red 640 ProbeType PCR WP2.1QuaL-Multiplex RealTime-PCRCytochromeB
PCR WP2.1QuaL-Multiplex RealTime-PCRCytochromeB Remarks X= labelling with LightCycler red 640. Cytochrome b. WP 2.1: Development of multiplex-PCR for species in food. Qualitative multiplex real time PCR for pig, cattle and myostatin on the Light Cycler, Roche.
Name: WP2.1QuaL-Multiplex RealTime-PCRCytochromeB Type: Real-Time PCR + Multiplex System: QuaLitative (Light Cycler; TaqMan; Matsunaga) Sequence: X56295
Genetic Element: Mitochondrial Cytochrome b Ampl.Length: 117 GenomSize: AnnTemp:
PPName Type PrimerSequence Length T_ann TmThermo Tm2AT %GC Position
cons_back_G Antisense gTCTg ATgTg TAgTg TATTg CTAgg AATAg g 31 68.1 59.4 88 41.9 144-174
cons_back_M Antisense gTCgg ATgTg TAgTg TATTg CTAgg AATAg g 31 71.3 62.5 90 45.2 144-174
SIM_F_iFL Sense gACCT CCCAg CTCCA TCAAA CATCT CATCT TgA X T gAA A 38 72.1 72.4 95 44.7 58-95
bos_probe_new Probe_Antisense CCCAg gAggg AACCg AAATT X 20 69.6 60.8 62 55 14607-14627
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