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| PCR |
| Name: |
LAC-border |
Type: |
Sequencing |
System: |
Sequencing |
| Genetic Element: |
Lactalbumin |
Ampl.Length: |
579 |
GenomSize: |
|
AnnTemp: |
|
| Taxonomy: |
Animals |
PCR Description: |
|
Temp. Time Program: |
|
| Device: |
|
Master Mix: |
|
nPrimers: |
3 |
Organisation: |
BfR |
| Validation: |
NoValidationAvailable |
Sequence: |
AB052166 |
| Remarks: |
Reference Sequence: AB052166.
PCR-type changed from PCR-RFLP to Sequencing. |
| Primers |
| PPName |
Type |
PrimerSequence |
Length |
T_ann |
TmThermo |
Tm2AT |
%GC |
Position |
|
LAC-border-f |
Sense |
CTgAT gACAT TATgT gTgTC AAgA |
24 |
60.9 |
52.2 |
66 |
37.5 |
1203-1226 |
|
LAC-border-r |
Antisense |
AgAgT gCTTT ATggg CCAA |
19 |
63.6 |
54.9 |
56 |
47.4 |
1781-1763 |
|
LAC-border-f |
Amplicon |
ctgat gacat tatgt gtgtc aagaa gattc tggat aaagt aggaa ttaac tactg gtgag tcacc tctct atttt tcact taatc tttcc tctct ttctt ctcag tcctt tcgtc ccagc actat actcc tttct ctcta tttcc tggtc tttta agcta gaatg taatc ttaaa aacaa aaatc atcaa gcaga ctccg gtttc caatt ttgaa gcttc actta cttca ctccc gttag caatt ttcct accta agggt cccta ataga gggct gagat ccagg atttc cttca ccagg acttg aacat ctaat tctac ttgtt cagtc ctaca tccta aggca cgccc tttga ccact gcccc gcaat tttct tggag tttta aaaaa tggac cttac tccac taagt ggctc agtgt ctcta gccat gtggc tagga aagtc tgtct gtaat tttaa cccac agtct tccac ctcag ccttc ctggg gataa agcta gatgt aaatc taacc aagat cctgt cagta atttg ccttg tctcc ttctt catga tcagg ttggc ccata aagca ctct |
579 |
|
92.8 |
1642 |
41.8 |
1203-1781 |
| Sequence |
| NCBI-Acc: |
AB052166 |
Sequence Description: |
Bos taurus gene for alpha lactalbumin, complete cds, strain:Holstein. |
| BaseCount: |
464 a 443 c 346 g 617 t |
| DNA-Sequence: |
AB052166.txt |
AminoAcid-Sequence: |
AB052166.txt |
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