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Primer

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Primer
PPName MY-r Type Antisense PrimerSequence ATACC AgTgC CTggg TTCAT
Length 20 T_ann 64.8 TmThermo 56.0 Tm2AT 60 %GC 50.0
Position 2576-2557 Sequence Dye ProbeType PCR MY
PCR MY Remarks Old position data: 605-586
PCR
Name: MY Type: Real-Time PCR System: QuaNtitative Sequence: AB076403
Genetic Element: Myostatin Ampl.Length: 97 GenomSize: AnnTemp: 60
Primers
PPName Type PrimerSequence Length T_ann TmThermo Tm2AT %GC Position
MY-f Sense TTgTg CAAAT CCTgA gACTC AT 22 63.8 55.1 62 40.9 2480-2501
MY-probe Marker/Probe CCCAT gAAAg ACggT ACAAg gTATA CTg 28 71.4 61.9 82 46.4 2506-2533
MY-Amplicon Amplicon TTGTG CAAAT CCTGA GACTC ATcaa aCCCA TGAAA GACGG TACAA GGTAT ACTGg aatcc gatct ctgaa acttg acATG AACCC AGGCA CTGGT AT 97 60 87 280 44.3 2480-2576
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