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PPName MY-Amplicon Type Amplicon PrimerSequence TTGTG CAAAT CCTGA GACTC ATcaa aCCCA TGAAA GACGG TACAA GGTAT ACTGg aatcc gatct ctgaa acttg acATG AACCC AGGCA CTGGT AT
Length 97 T_ann 60 TmThermo 87 Tm2AT 280 %GC 44.3
Position 2480-2576 Sequence Dye ProbeType PCR MY
PCR MY Remarks
Name: MY Type: Real-Time PCR System: QuaNtitative Sequence: AB076403
Genetic Element: Myostatin Ampl.Length: 97 GenomSize: AnnTemp: 60
PPName Type PrimerSequence Length T_ann TmThermo Tm2AT %GC Position
MY-f Sense TTgTg CAAAT CCTgA gACTC AT 22 63.8 55.1 62 40.9 2480-2501
MY-r Antisense ATACC AgTgC CTggg TTCAT 20 64.8 56.0 60 50.0 2576-2557
MY-probe Marker/Probe CCCAT gAAAg ACggT ACAAg gTATA CTg 28 71.4 61.9 82 46.4 2506-2533
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