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Name: MY Type: Real-Time PCR System: QuaNtitative
Genetic Element: Myostatin Ampl.Length: 97 GenomSize: AnnTemp: 60
Taxonomy: Animals PCR Description: Temp. Time Program:
Device: Master Mix: nPrimers: 4 Organisation: BfR
Validation: NoValidationAvailable Sequence: AB076403
Remarks: Reference Sequence: AB076403 / AB019620 Publication: Laube, I., Butschke, A., Zagon, J., Spiegelberg, A., Schauzu, M., Bögl, K.-W., Kroh, L. W., Broll, H., 2001. Nachweisverfahren für Rindfleisch in Lebensmitteln unter Anwendung der TaqMan-Technologie. Bundesgesundheitsblatt Gesundheitsforsch. Gesundheitsschutz
PPName Type PrimerSequence Length T_ann TmThermo Tm2AT %GC Position
MY-f Sense TTgTg CAAAT CCTgA gACTC AT 22 63.8 55.1 62 40.9 2480-2501
MY-r Antisense ATACC AgTgC CTggg TTCAT 20 64.8 56.0 60 50.0 2576-2557
MY-probe Marker/Probe CCCAT gAAAg ACggT ACAAg gTATA CTg 28 71.4 61.9 82 46.4 2506-2533
MY-Amplicon Amplicon TTGTG CAAAT CCTGA GACTC ATcaa aCCCA TGAAA GACGG TACAA GGTAT ACTGg aatcc gatct ctgaa acttg acATG AACCC AGGCA CTGGT AT 97 60 87 280 44.3 2480-2576
NCBI-Acc: AB076403 Sequence Description: Bos taurus gene for myostatin, complete cds.
BaseCount: 2196 a 1116 c 1139 g 2209 t
DNA-Sequence: AB076403.txt AminoAcid-Sequence: AB076403.txt
Short Title Type Date Download
Rep: Beef_TaqMan Report 2001
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