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PPName cons_back_M Type Antisense PrimerSequence gTCgg ATgTg TAgTg TATTg CTAgg AATAg g
Length 31 T_ann 71.3 TmThermo 62.5 Tm2AT 90 %GC 45.2
Position 144-174 Sequence X56295 Dye ProbeType PCR WP2.1QuaL-Multiplex RealTime-PCRCytochromeB
PCR WP2.1QuaL-Multiplex RealTime-PCRCytochromeB Remarks Cytochrome b (Amplicon length: 117bp). WP 2.1: Development of multiplex-PCR for species in food. Qualitative multiplex real time PCR for pig, cattle and myostatin on the Light Cycler, Roche.
Name: WP2.1QuaL-Multiplex RealTime-PCRCytochromeB Type: Real-Time PCR + Multiplex System: QuaLitative (Light Cycler; TaqMan; Matsunaga) Sequence: X56295
Genetic Element: Mitochondrial Cytochrome b Ampl.Length: 117 GenomSize: AnnTemp:
PPName Type PrimerSequence Length T_ann TmThermo Tm2AT %GC Position
cons_back_G Antisense gTCTg ATgTg TAgTg TATTg CTAgg AATAg g 31 68.1 59.4 88 41.9 144-174
SIM_F_iFL Sense gACCT CCCAg CTCCA TCAAA CATCT CATCT TgA X T gAA A 38 72.1 72.4 95 44.7 58-95
bos_probe_new Probe_Antisense CCCAg gAggg AACCg AAATT X 20 69.6 60.8 62 55 14607-14627
sus_probe Probe_Antisense CAgAT gCCTA AgAgg gAACC gA X 22 69.3 60.6 68 54.5 98-119
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