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Primer

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Primer
PPName MY-f Type Sense PrimerSequence TTgTg CAAAT CCTgA gACTC AT
Length 22 T_ann 63.8 TmThermo 55.1 Tm2AT 62 %GC 40.9
Position 2480-2501 Sequence Dye ProbeType PCR MY
PCR MY Remarks Old position data: 509-530
PCR
Name: MY Type: Real-Time PCR System: QuaNtitative Sequence: AB076403
Genetic Element: Myostatin Ampl.Length: 97 GenomSize: AnnTemp: 60
Primers
PPName Type PrimerSequence Length T_ann TmThermo Tm2AT %GC Position
MY-r Antisense ATACC AgTgC CTggg TTCAT 20 64.8 56.0 60 50.0 2576-2557
MY-probe Marker/Probe CCCAT gAAAg ACggT ACAAg gTATA CTg 28 71.4 61.9 82 46.4 2506-2533
MY-Amplicon Amplicon TTGTG CAAAT CCTGA GACTC ATcaa aCCCA TGAAA GACGG TACAA GGTAT ACTGg aatcc gatct ctgaa acttg acATG AACCC AGGCA CTGGT AT 97 60 87 280 44.3 2480-2576
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