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abrunden

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abrunden

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Primer

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Primer
PPName MY-probe Type Marker/Probe PrimerSequence CCCAT gAAAg ACggT ACAAg gTATA CTg
Length 28 T_ann 71.4 TmThermo 61.9 Tm2AT 82 %GC 46.4
Position 2506-2533 Sequence Dye 6FAM ProbeType TaqMan PCR MY
PCR MY Remarks Old position data: 535-562; Old Seq: Last base: [2534]: T => Length: 29 (?)
PCR
Name: MY Type: Real-Time PCR System: QuaNtitative Sequence: AB076403
Genetic Element: Myostatin Ampl.Length: 97 GenomSize: AnnTemp: 60
Primers
PPName Type PrimerSequence Length T_ann TmThermo Tm2AT %GC Position
MY-f Sense TTgTg CAAAT CCTgA gACTC AT 22 63.8 55.1 62 40.9 2480-2501
MY-r Antisense ATACC AgTgC CTggg TTCAT 20 64.8 56.0 60 50.0 2576-2557
MY-Amplicon Amplicon TTGTG CAAAT CCTGA GACTC ATcaa aCCCA TGAAA GACGG TACAA GGTAT ACTGg aatcc gatct ctgaa acttg acATG AACCC AGGCA CTGGT AT 97 60 87 280 44.3 2480-2576
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