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Taxonomy
Popular Name: Genus: Species: Class: Order: Family:
Animals
Sequence
NCBI-Acc: AB076403 Sequence Description: Bos taurus gene for myostatin, complete cds.
BaseCount: 2196 a 1116 c 1139 g 2209 t
DNA-Sequence: AB076403.txt AminoAcid-Sequence: AB076403.txt
PCR
Name: MY Type: Real-Time PCR System: QuaNtitative Sequence: AB076403
Genetic Element: Myostatin Ampl.Length: 97 GenomSize: AnnTemp: 60
Primers
PPName Type PrimerSequence Length T_ann TmThermo Tm2AT %GC Position
MY-f Sense TTgTg CAAAT CCTgA gACTC AT 22 63.8 55.1 62 40.9 2480-2501
MY-r Antisense ATACC AgTgC CTggg TTCAT 20 64.8 56.0 60 50.0 2576-2557
MY-probe Marker/Probe CCCAT gAAAg ACggT ACAAg gTATA CTg 28 71.4 61.9 82 46.4 2506-2533
MY-Amplicon Amplicon TTGTG CAAAT CCTGA GACTC ATcaa aCCCA TGAAA GACGG TACAA GGTAT ACTGg aatcc gatct ctgaa acttg acATG AACCC AGGCA CTGGT AT 97 60 87 280 44.3 2480-2576
Publications
Short Title Type Date Download
Rep: Beef_TaqMan Report 2001
Organisation Data
Acronym: BfR BfR Phone: +49-30-8412-0
Partner Number: 1 Fax: +41 308412 3685
Type: Authority e-Mail: j.zagon@bfr.bund.de
Name: BfR-Federal Institute for Risk Assessment, Berlin Website: http://www.bfr.bund.de/
Umbrella Organisation: Bund, Germany (Bundesinstitut für Risikobewertung)
Postal Address: Thielallee 88-92, D-14195 Berlin Country: Germany
Remarks: Coordinator MolSpec-ID-project, Task Leader Workpackages 1.4 and 4
Persons
Title / Name e-Mail Phone Remarks
Mr. Hermann Broll h.broll@bfr.bund.de +49-30-8412 3639
Mr. Andreas Butschke a.butschke@bfr.bund.de +49-30-8412 3687
Dr. Jutta Zagon j.zagon@bfr.bund.de +49-30-8412 3876 MolSpec-Coordinator
Samples
FoodName Extraction Process cNucl cDNA aDNA Supplier
Muscle meat from 7 different animal species NucleoSpinTissue NoData 5 - 69 3 BFEL
Muscle meat from 7 different animal species InviSorb NoData 22 - 61 3 BFEL
Muscle meat from 7 different animal species Dneasy NoData 15 - 79 3 BFEL
Muscle meat from 7 different animal species NucleoSpinFood NoData 26 - 132 3 BFEL
Muscle meat from 7 different animal species E.Z.N.A NoData 4 - 26 3 BFEL
Muscle meat from 7 different animal species Lyse NoData 6 - 7 3 BFEL
Muscle meat from 7 different animal species CTAB NoData 83 - 306 3 BFEL
Muscle meat from 7 different animal species Triton-X100 NoData 8 - 23 2 BFEL
Salami (S2 from Partner 2, no heat treatment) CTAB no heat treatment 52±8 80±16 103 VUP
Salami (S2 from Partner 2, no heat treatment) GeneSpin no heat treatment 22±1 42±10 103 VUP
Salami (S2 from Partner 2, no heat treatment) InviSorb no heat treatment 106±17 222±69 104 VUP
Pâté (S3 from Partner 2, heat-treated at 117 °C) CTAB heat-treated at 117 °C 28±7 97±15 102 VUP
Pâté (S3 from Partner 2, heat-treated at 117 °C) GeneSpin heat-treated at 117 °C 6±2 8±7 100 VUP
Pâté (S3 from Partner 2, heat-treated at 117 °C) InviSorb heat-treated at 117 °C 40±13 270±34 102 VUP
Devices
Electrophor. RT-device DNA-Seqencer ThermoCyl. SouthernBlot Other
DNA+protein ABI 7700+LC ABI 310 PE 2400 yes ELISA plate reader
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