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PPName Capra-12S-f Type Sense PrimerSequence TgggC TACAT TTTCT ACCTT AAg
Length 23 T_ann 62.4 TmThermo 53.7 Tm2AT 64 %GC 39.1
Position 779-801 Sequence Dye ProbeType PCR Capra-12S
PCR Capra-12S Remarks
Name: Capra-12S Type: PCR-RFLP System: QuaLitative (Light Cycler; TaqMan; Matsunaga) Sequence: NC_005044
Genetic Element: 12S rRNA (?) Ampl.Length: 179 GenomSize: 3.24 AnnTemp: 54
PPName Type PrimerSequence Length T_ann TmThermo Tm2AT %GC Position
Capra-12S-r Antisense CgTgT TAATA ggCTT gAgTg C 21 63.2 54.4 62 47.6 957-937
Capra-12S-Amplicon Amplicon TGGGC TACAT TTTCT ACCTT AAGaa aatta atacg aaagc catta tgaaa ttaat gacca aagga ggatt tagta gtaaa ctaag aatag agtgc ttagt tgaat taggc catga agcac gcaca caccg cccgt caccc tcctc aagta aatac aatGC ACTCA AGCCT ATTAA CACG 179 54 88.3 498 39.1 779-957
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