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PPName Capra-12S-Amplicon Type Amplicon PrimerSequence TGGGC TACAT TTTCT ACCTT AAGaa aatta atacg aaagc catta tgaaa ttaat gacca aagga ggatt tagta gtaaa ctaag aatag agtgc ttagt tgaat taggc catga agcac gcaca caccg cccgt caccc tcctc aagta aatac aatGC ACTCA AGCCT ATTAA CACG
Length 179 T_ann 54 TmThermo 88.3 Tm2AT 498 %GC 39.1
Position 779-957 Sequence Dye ProbeType PCR Capra-12S
PCR Capra-12S Remarks
Name: Capra-12S Type: PCR-RFLP System: QuaLitative (Light Cycler; TaqMan; Matsunaga) Sequence: NC_005044
Genetic Element: 12S rRNA (?) Ampl.Length: 179 GenomSize: 3.24 AnnTemp: 54
PPName Type PrimerSequence Length T_ann TmThermo Tm2AT %GC Position
Capra-12S-f Sense TgggC TACAT TTTCT ACCTT AAg 23 62.4 53.7 64 39.1 779-801
Capra-12S-r Antisense CgTgT TAATA ggCTT gAgTg C 21 63.2 54.4 62 47.6 957-937
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