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Name: Capra-12S Type: PCR-RFLP System: QuaLitative (Light Cycler; TaqMan; Matsunaga)
Genetic Element: 12S rRNA (?) Ampl.Length: 179 GenomSize: 3.24 AnnTemp: 54
Taxonomy: Goat PCR Description: Temp. Time Program:
Device: Master Mix: nPrimers: 3 Organisation: BfR
Validation: NoValidationAvailable Sequence: NC_005044
Remarks: Reference Sequence: NC_005044 Taxon: Only Capra hircus
PPName Type PrimerSequence Length T_ann TmThermo Tm2AT %GC Position
Capra-12S-f Sense TgggC TACAT TTTCT ACCTT AAg 23 62.4 53.7 64 39.1 779-801
Capra-12S-r Antisense CgTgT TAATA ggCTT gAgTg C 21 63.2 54.4 62 47.6 957-937
Capra-12S-Amplicon Amplicon TGGGC TACAT TTTCT ACCTT AAGaa aatta atacg aaagc catta tgaaa ttaat gacca aagga ggatt tagta gtaaa ctaag aatag agtgc ttagt tgaat taggc catga agcac gcaca caccg cccgt caccc tcctc aagta aatac aatGC ACTCA AGCCT ATTAA CACG 179 54 88.3 498 39.1 779-957
NCBI-Acc: NC_005044 Sequence Description: Capra hircus mitochondrion, complete genome.
BaseCount: 5569 a 4313 c 2189 g 4569 t
DNA-Sequence: NC_005044.txt AminoAcid-Sequence: NC_005044.txt
Short Title Type Date Download
SOP: Goat_QuaL/SemiQuaNt SOP 2004 SOP_goat_beta_casein_p6.pdf
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