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Primer
PPName MY-r-long Type Antisense PrimerSequence CCAAA TACCA gTgCC TgggT TCA
Length 23 T_ann 72.1 TmThermo 63.4 Tm2AT 70 %GC 52.2
Position 2558-2580 Sequence AB076403 Dye ProbeType PCR WP2.1 QuaL-Multiplex RealTime-PCR Myostatin
PCR WP2.1 QuaL-Multiplex RealTime-PCR Myostatin Remarks Myostatin: Amplicon length: 99bp. WP 2.1:Development of multiplex-PCR for species in food. Qualitative multiplex real time PCR for pig, cattle and myostatin on the Light Cycler, Roche.
PCR
Name: WP2.1 QuaL-Multiplex RealTime-PCR Myostatin Type: Real-Time PCR + Multiplex System: QuaLitative (Light Cycler; TaqMan; Matsunaga) Sequence: AB076403
Genetic Element: Myostatin Ampl.Length: 99 GenomSize: AnnTemp:
Primers
PPName Type PrimerSequence Length T_ann TmThermo Tm2AT %GC Position
MY-f_iFL Sense TTgTg CAAAT CCTgA gAC X T CAT 22 63.8 55.5 62 40.9 2480-2501
LC-705_Myo Probe_Antisense ACCTT gTACC gTCTT TCATg ggTTT X 25 70.8 62.3 72 44 2503-2527
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