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Primer
PPName LC-705_Myo Type Probe_Antisense PrimerSequence ACCTT gTACC gTCTT TCATg ggTTT X
Length 25 T_ann 70.8 TmThermo 62.3 Tm2AT 72 %GC 44
Position 2503-2527 Sequence AB076403 Dye LightCycler red 705 ProbeType PCR WP2.1 QuaL-Multiplex RealTime-PCR Myostatin
PCR WP2.1 QuaL-Multiplex RealTime-PCR Myostatin Remarks X= labelling with LightCycler red 705 Myostatin: WP 2.1:Development of multiplex-PCR for species in food. Qualitative multiplex real time PCR for pig, cattle and myostatin on the Light Cycler, Roche.
PCR
Name: WP2.1 QuaL-Multiplex RealTime-PCR Myostatin Type: Real-Time PCR + Multiplex System: QuaLitative (Light Cycler; TaqMan; Matsunaga) Sequence: AB076403
Genetic Element: Myostatin Ampl.Length: 99 GenomSize: AnnTemp:
Primers
PPName Type PrimerSequence Length T_ann TmThermo Tm2AT %GC Position
MY-r-long Antisense CCAAA TACCA gTgCC TgggT TCA 23 72.1 63.4 70 52.2 2558-2580
MY-f_iFL Sense TTgTg CAAAT CCTgA gAC X T CAT 22 63.8 55.5 62 40.9 2480-2501
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