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Name: WP2.1 QuaL-Multiplex RealTime-PCR Myostatin Type: Real-Time PCR + Multiplex System: QuaLitative (Light Cycler; TaqMan; Matsunaga)
Genetic Element: Myostatin Ampl.Length: 99 GenomSize: AnnTemp:
Taxonomy: Animals PCR Description: WP 2.1: Development of multiplex-PCR for species in foods. Qualitative multiplex real time PCR for pig, cattle and myostatin on the Light Cycler, Roche. Target Element: Myostatin Temp. Time Program: PCR profile Taq Activation 95 °C 600 sec Cycles 38 Denat. Amplif. Extension ------------------------------------------------- Target Temp. (°C) 95 64 72 Incubation time (sec) 5 5 4 Temp. Transition Rate (°C/sec) 20.0 20.0 20.0 Secondary target temp. (°C) 0 60 0 Step size (°C) 0.0 1.0 0 Step delay (Cycles) 0 10 0 Acquisition mode None Single None
Device: Light Cycler, Roche Master Mix: nPrimers: Organisation: TIB
Validation: Sequence: AB076403
Remarks: Detection of Cattle (66), and maybe also Pig (79) Target Element: Myostatin
PPName Type PrimerSequence Length T_ann TmThermo Tm2AT %GC Position
MY-r-long Antisense CCAAA TACCA gTgCC TgggT TCA 23 72.1 63.4 70 52.2 2558-2580
MY-f_iFL Sense TTgTg CAAAT CCTgA gAC X T CAT 22 63.8 55.5 62 40.9 2480-2501
LC-705_Myo Probe_Antisense ACCTT gTACC gTCTT TCATg ggTTT X 25 70.8 62.3 72 44 2503-2527
NCBI-Acc: AB076403 Sequence Description: Bos taurus gene for myostatin, complete cds.
BaseCount: 2196 a 1116 c 1139 g 2209 t
DNA-Sequence: AB076403.txt AminoAcid-Sequence: AB076403.txt
Short Title Type Date Download
Rep: WP2.1_MultiplexPCR_TibMolbiol Report 2004 Rep_WP2.1_MultiplexPCR_TibMolbiol.pdf
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