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PCR

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PCR

Name:

GDF8-SPE

Type: PCR-RFLP System:
Genetic Element: Ampl. Length: Ampl. Position: Sequence Size [bp]:
AnnTemp [°C]: Taxonomy: Cattle (Beef (general)) Temp. Time Program: Cycling conditions :<br>One cycle of DNA denaturation for 2 min at 94 °C;<br>35 three-step cycles 94 °C for 1 min<br>56 °C for 1 min<br>and 72 °C for 1 min 30 s<br>Last extension for 10 min at 72 °
Device: Reagents: Primer 1 (10 &#956;M) 4 &#956;l<br>Primer 2 (10 &#956;M) 4 &#956;l<br>PCR buffer 10x 5 &#956;l<br>MgCl2 (25 mM) 3 &#956;l<br>dNTPs (2 mM) 2,5 &#956;l<br>Taq DNA polymerase (5 U/&#956;l) 0,4 &#956;l<br>H2O<br>ADN 100 ng<br>Total volume 50 &#956;l nPrimers: 2 Organisation: CRA-W
PCR Description: PCR-RFLP protocol used for the genotyping of the Myostatin promoter according to Crisà et al. (2003) Sequence:
LOD [copy]: LOQ [copy]: Efficiency [%]:
Remarks: PCR-RFLP protocol used for the genotyping of the Myostatin promoter according to Crisà et al. (2003)

Food/Product

Food Name: Generic Image: Product Type: Food Source: Product Website:
Beef meatMEAT AND MEAT PRODUCTS [A0459]COW [B1201]http://europa.e... l/en/501_en.htm

Primers

PPName: Type: PrimerSequence: Length [bp]: T_Ann [°C]: TmThermo [°C]: Tm2AT [°C]: GC [%]: Position:
GDF8-SPE-F Sense gCT CCC AgA CCT TAC CCC AAA TC 23 71.2 62.4 72 56.52
GDF8-SPE-R Antisense gTT gAT ATg CTT TTC CCT CAg 21 61.1 52.3 60 42.86

Publications (more...)

Short Title Type Date Download
PCR-RFLP protocol of the Myostatin promoter SOP September 2007 SOP_CRA-W_genotypes_CoatColourGenes_071016.pdf

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