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You are here: Core Forms > Search PCR > PCR

PCR

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Entry No. of 51 

PCR

Name:

CerQuant

Type: Real-Time PCR System: Quantitative
Genetic Element: Ampl. Length: Ampl. Position: Sequence Size [bp]:
AnnTemp [°C]: Taxonomy: Cereals: Oat-Barley-Rye-Wheat Temp. Time Program: 1 X<br>95°C 5 min<br>95°C 10 sec ; acquisition mode "none"<br>45 X<br>57°C 60 sec ; acquisition mode "single"<br>40°C 30 sec
Device: Reagents: Reagents Vol/PCR<br>(&#956;L)<br>H2O 7.15<br>PCR Buffer Takara (10X) 2<br>MgCl2 (25mM) 1.2<br>BSA (0.02&#956;g/&#956;L) 2<br>dNTP (2.5mM) 2<br>Primer Cer1 F (20&#956;M) 0.9<br>Primer Cer2R (20&#956;M) 0.9<br>Probe fluoCer1 (20&#956;M) 0.8<br>Probe redCer1-APD (20&#956;M) 0.8<br>TaqPol Takara (5U/&#956;l) 0.25<br>Volume total 18 &#956;L<br>DNA : 2 &#956;L nPrimers: 4 Organisation: Eurofins
PCR Description: Sequence:
LOD [copy]: LOQ [copy]: Efficiency [%]:
Remarks:

Food/Product

Food Name: Generic Image: Product Type: Food Source: Product Website:
WheatCereals and cereal products [A0457]

Primers

PPName: Type: PrimerSequence: Length [bp]: T_Ann [°C]: TmThermo [°C]: Tm2AT [°C]: GC [%]: Position:
Cer1F Sense AAT CCT gAg CCA AAT CCg Tg 20 66.9 58.1 60 50.00
Cer2R Antisense CAT gTA gAA Tgg gAC TCT CTC TTT g 25 64.8 56.0 72 44.00
fluoCER1 Marker/Probe 5&q uot ; T AAC TCg ATT CgT TAg AAC AgC TTC CAT TgA gTC TCT -- FL 37
redCER1_APD Marker/Probe 5&q uot ; L C R ed6 40 - C ACC TAT CCT TTT CCT TTg TAT TCT AgT TCg AgA — PH 34

Publications (more...)

Short Title Type Date Download
SOP for the Quantitative Detection of Oat SOP 03.08.2007 SOP_Eurofins_Oat_quantitative_PCR_070803.pdf

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