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You are here: Core Forms > Search PCR > PCR

PCR

Entry No. of 51 
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PCR

Name:

Bras_lip

Type: Real-Time PCR System: Quantitative
Genetic Element: Lipase 1 Ampl. Length: 122 Ampl. Position: Sequence Size [bp]:
AnnTemp [°C]: 57 Taxonomy: Rape, Canola, Rapeseed, Oilseed Rape, Colza Temp. Time Program: Process Time [min:s] Temperature [°C]<br>Pre-PCR: decontamination 02:00 50<br>Pre-PCR: activation of DNA polymerase and denaturation of template DNA 10:00 95<br>PCR (45 cycles) <br>Step 1 Denaturation 00:15 95<br>Step 2 Annealing and elongation 01:00 60
Device: Reagents: nPrimers: 3 Organisation: BfR
PCR Description: Sequence: AY866419
LOD [copy]: 1 LOQ [copy]: 10 Efficiency [%]: 102
Remarks:

Food/Product

Food Name: Generic Image: Product Type: Food Source: Product Website:
Miel de CorseSUGAR AND HONEY [A0462]INSECT [B1220]http://europa.e... /en/1129_en.htm

Primers

PPName: Type: PrimerSequence: Length [bp]: T_Ann [°C]: TmThermo [°C]: Tm2AT [°C]: GC [%]: Position:
Bras_lip-f2 Sense CAC gAC CAC gTT CTT TgT TTT 21 66.0 (PCR: 57) 57.3 60 42.86
Bras_lip-r Antisense CCA CTA CAA CAT TCC ATC CCC 21 66.0 (PCR: 57) 57.2 64 52.38
Bras_lip-probe Marker/Probe 5Ž -FA M)- TC ACC AAA ACC gCA gCA AgC A - (TA MRA )-3 Ž 21 72.1 (PCR: 57) 65.4 64 52.38

Sequence (more...)

NCBI-Acc:

AY866419

Sequence Description: Brassica napus lipase 1 mRNA, complete cds.
BaseCount:
DNA-Sequence: AminoAcid-Sequence:

Publications (more...)

Short Title Type Date Download
SOP PlantSpecies by PCR SOP September 2007 SOP_BfR_species-detection_071016.pdf
Analytical Methods: Detection of plant species in honey Journal Accepted: 22.09.2008 FoodChemistry_118_979-986_Laube_Honey_2010.pdf

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