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Publication

Short Title:

SOP real-time PCR for Apis species

Full Title:

Detection of different Apis species with real-time PCR

Authors: Mandy Schoene-Michling Organisation: Biolytix
Publication Date: November 2005 Version: Publication Type: SOP Language: English
ISBN: ISSN: Report Title:
Volume: Issue: Pages: 4 Download: Detection-ApisSpecies-Conf.pdf
Medium: PRINT Method: URL: Problem with Link:
Remarks: Detection-ApisSpecies-Public.pdf

PCR

Name: Type: System: Ampl.Length: Sequence Size [bp]: AnnTemp [°C]:
ApisCerana_RT Real-Time PCR Qualitative 113 470.Apis cerana mitochondr.16S rRNA large subunit 57-62
ApisDorsata_RT Real-Time PCR Qualitative 101 462.Apis dorsana mitochondr.16S rRNA large subunit 55-59
ApisFlorea_RT Real-Time PCR Qualitative 105 469.Apis cerana mitochondr.16S rRNA large subunit 52-57
ApisMellifera_RT Real-Time PCR Qualitative 103 467.Apis mellifera mitochondr.16S rRNA large subun 55-61
Taxonomy
Popular Name: Genus: Species: Class: Order: Family:
Oriental Honeybee Apis cerana Insecta Hymenoptera Apidae
Rock Honeybee Apis dorsata Insecta Hymenoptera Apidae
Dwarf Honeybee Apis florea Insecta Hymenoptera Apidae
Bee Apis mellifera Insecta Hymenoptera Apidae
Greenbug Schizaphis graminum Insecta Hemiptera Aphididae
Greenbug Bacteria Buchnera aphidicola Gammaproteobacteria Enterobacteriales Enterobacteriaceae

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